Document 2746
 DOCN  M94A2746
 TI    Quantitation and prevalence of HIV RNA in Japanese HIV carriers and AIDS
       patients.
 DT    9412
 AU    Ohno T; Hayashi T; Saito T; Ito A; Nagao T; Akagi K; Imai M; Kanagawa
       Pref. Inst. of Health, Japan.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):234 (abstract no. PB0366). Unique
       Identifier : AIDSLINE ICA10/94369829
 AB    OBJECTIVE: Clinical relevance of HIV RNA positive rate and quantitative
       measurement of HIV RNA in Japanese HIV carriers and AIDS patients in
       various disease stages was determined using a quantitative HIV RNA assay
       employing the bDNA probe method (bDNA HIV). METHODS: A total of 68
       subjects, who were anti-HIV positive ranging from Stage II(asymptomatic)
       to Stage IV C2 in the CDC classification, were selected for the study.
       bDNA HIV was run on the plasma specimens collected from those subjects
       and compared with P24 antigen test and HIV isolation in cell culture.
       Quantitation of HIV RNA was also carried out in the patients treated
       with AZT and ddI. RESULTS: Among 68 HIV infected cases, positive rate of
       HIV RNA, viral isolation and P24 antigen was 54.2%, 47.2% and 25.8%,
       respectively. Respective Positive rate of HIV RNA in Stage II and Stage
       IV C1 patients was 45.1% and 66.1%. The level of HIV RNA increased as
       the disease progressed and decreased after AZT or ddI treatment despite
       no significant changes observed by P24 antigen test. DISCUSSION AND
       CONCLUSIONS: Monitoring the prevalence of HIV RNA and quantitating HIV
       RNA in the HIV infected cases may be very significant in understanding
       of the progression of the disease or in evaluation of antiviral
       treatment.
 DE    Acquired Immunodeficiency Syndrome/CLASSIFICATION/*DIAGNOSIS/DRUG
       THERAPY  Didanosine/THERAPEUTIC USE  DNA Probes/DIAGNOSTIC USE  Human
       HIV/*GENETICS  HIV Infections/CLASSIFICATION/*DIAGNOSIS/DRUG THERAPY
       HIV Seropositivity/CLASSIFICATION/*DIAGNOSIS/DRUG THERAPY  HIV
       Seroprevalence  Japan  RNA, Viral/*BLOOD  Zidovudine/THERAPEUTIC USE
       MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).