Document 2759
 DOCN  M94A2759
 TI    Diagnosis of HIV-1 infection with urine.
 DT    9412
 AU    Hashinaka K; Hashida S; Saitoh A; Shinagawa H; Takamizawa A; Oka S;
       Shimada K; Ishikawa E; Department of Biochemistry, Medical College of
       Miyazaki, Japan.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):231 (abstract no. PB0354). Unique
       Identifier : AIDSLINE ICA10/94369816
 AB    OBJECTIVE: To develop and test an enzyme immunoassay for diagnosis of
       HIV-1 infection by detection of anti-HIV-1 IgG in urine. METHOD: An
       ultrasensitive enzyme immunoassay (immune complex transfer enzyme
       immunoassay) using recombinant reverse transcriptase (R-RT) as antigen
       was developed. The immune complex consisting of 2,4-dinitrophenyl
       (DNP)-bovine serum albumin-R-RT conjugate, anti-HIV-1 IgG and
       R-RT-beta-D-galactosidase (GAL) conjugate was trapped onto anti-DNP
       IgG-coated polystyrene balls (PS), eluted with DNP-L-lysine and
       transferred to (anti-hIgG gamma-chain) IgG-coated PS. GAL activity was
       assayed by fluorometry. RESULTS: Using 100 microliters of urine, the
       lowest signals among 60 asymptomatic carriers, 11 patients with ARC and
       12 patients with AIDS were 14-, 29- and 10-fold, respectively, higher
       than the highest signal among 100 seronegatives. When urine was
       concentrated, signals were proportionally enhanced for seropositives but
       not significantly increased for seronegatives. Anti-HIV-1 IgG in urine
       was stable at -20 degrees C for at least 6 months. CONCLUSION: From the
       above results, the sensitivity and specificity were expected to be both
       100% even for a larger number of cases, although the number of cases
       tested was limited.
 DE    beta-Galactosidase/ANALYSIS  Acquired Immunodeficiency Syndrome/URINE
       AIDS-Related Complex/URINE  Dinitrophenols  Evaluation Studies  Human
       HIV Antibodies/*URINE  HIV Infections/*DIAGNOSIS/URINE
       HIV-1/*IMMUNOLOGY  IgG/IMMUNOLOGY/*URINE  *Immunoenzyme Techniques
       Microspheres  Recombinant Proteins/ANALYSIS/IMMUNOLOGY  Reverse
       Transcriptase/IMMUNOLOGY  Sensitivity and Specificity  Serum Albumin,
       Bovine  MEETING ABSTRACT

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