Document 2760
 DOCN  M94A2760
 TI    Salivary HIV antibody testing: is it accurate?
 DT    9412
 AU    Cook D; King AS; Marion SA; Montaner J; Rekart M; Middleton P;
       O'Shaughnessy M; University of British Columbia, Vancouver, Canada.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):230 (abstract no. PB0350). Unique
       Identifier : AIDSLINE ICA10/94369815
 AB    OBJECTIVES: To compare the accuracy of a saliva test protocol in
       determining the HIV-1 antibody status of individuals in various clinical
       settings and stages of HIV disease. METHODS: Paired samples of serum and
       saliva were obtained from 1256 subjects at four study sites in
       Vancouver: 337 from a hospital-based infectious diseases clinic serving
       HIV seropositive patients, 478 from an AIDS testing clinic, 10 from an
       outpatient hemophilia clinic and 431 from a street-based nurse outreach
       program. Serum was tested using standard methods. Saliva was tested
       using Recombigen HIV-1 EIA (Cambridge Biotech). Reactive saliva EIA
       tests were retested singly and further tested by
       radioimmunoprecipitation (RIPA). RESULTS: There were 368 seropositive
       and 888 seronegative subjects. HIV antibodies were detected in saliva as
       follows: TABULAR DATA, SEE ABSTRACT VOLUME. HIV antibodies were not
       detected in saliva from seronegative individuals (specificity = 100%).
       DISCUSSION AND CONCLUSIONS: This study demonstrated good agreement
       between the results obtained from saliva and serum testing for HIV
       antibodies. Because the sensitivity of saliva testing is lower than for
       serum, it cannot be recommended for diagnostic testing but should prove
       to be a useful tool for epidemiologic purposes.
 DE    British Columbia/EPIDEMIOLOGY  Comparative Study  Evaluation Studies
       Human  HIV Antibodies/*ANALYSIS/BLOOD  HIV
       Infections/*DIAGNOSIS/EPIDEMIOLOGY  HIV-1/*IMMUNOLOGY  Immunoenzyme
       Techniques  Reagent Kits, Diagnostic  Saliva/*IMMUNOLOGY  Salivary
       Proteins/*ANALYSIS  Sensitivity and Specificity  MEETING ABSTRACT
       MULTICENTER STUDY

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