Document 2762
 DOCN  M94A2762
 TI    Evaluation of saliva test in HIV infection in Rosario, Argentina.
 DT    9412
 AU    Taborda M; Fuster L; de Rueda M; Bortolozzi R; Lupo S; Fay O; Center for
       technology in Public Health, National University of; Rosario, Argentina.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):230 (abstract no. PB0351). Unique
       Identifier : AIDSLINE ICA10/94369813
 AB    OBJECTIVE: To evaluate diagnostic usefulness of a saliva collection
       device detecting HIV 1-2 antibody. To compare the sensibility and
       specificity obtained between samples of saliva and serum. To know the
       relation between HIV 1-2 Ab presence or absence in saliva and clinical
       status HIV infected patients. MATERIAL AND METHODS: We were able to
       study 148 adult patients. Group I were 90 (61%) HIV(+) patients with
       different clinical status and group II were 58 (39%) people with
       spontaneous presentation to our Center in order to know their
       serological status. In 20 (22%) patients with AIDS from group I we were
       able to collect once a month during 3 months saliva and blood samples.
       Omni-sal (Saliva Inc. M.R.) were used to obtain saliva samples. Organon
       Teknika and Abbott laboratory Elisa test were used for screening and
       Western blot and LiaTek from Organon were used for confirmatory test. We
       follow the changes suggested by the manufacturer for the saliva samples
       process (sample volume, a-IgA peroxidase conjugate). RESULTS: In the
       group I the saliva and serum correlation was 96,4% (87(+)/90(+)). 10
       (11.49%) out of 87 EIA(+) samples were Western blot indeterminate(we
       used the ARC criteria of positivity) showed generally anti-env Antibody.
       3(-) saliva samples were indeterminate Western blot (showed gp41 gp120
       gp160) and correspond with the AIDS patient 3th samples. In the group II
       patients, the saliva and serum correlation were 96.5% (8(+)/10(+);
       48(-)/48(-)). The two negative samples were indeterminate in serum
       (Western blot (1) gp41 gp120 gp160 (2) p24) and HIV(+) partners with
       seroconversion. CONCLUSIONS: The test shows a low sensitivity in the
       adult population tested. 2) The mayor differences have been detected
       during the seroconversion period. 3) In AIDS patients with low
       antibodies titles, whereas Western blot remain indeterminate, saliva
       test became negative. 4) More extensive study must be done, particularly
       regarding screening among at risk population.
 DE    Adult  Argentina/EPIDEMIOLOGY  Blotting, Western  Comparative Study
       Enzyme-Linked Immunosorbent Assay  Evaluation Studies  Human  HIV
       Antibodies/*ANALYSIS/BLOOD  HIV Infections/*DIAGNOSIS/EPIDEMIOLOGY
       HIV-1/*IMMUNOLOGY  HIV-2/*IMMUNOLOGY  Sensitivity and Specificity
       MEETING ABSTRACT

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