Document 2768
 DOCN  M94A2768
 TI    Searching for anti-HIV antibodies in saliva samples.
 DT    9412
 AU    Hurtado LV; Andrade Arzabe R; Camacho M; Zegarra L; Torrico L; Von Poser
       B; Inmunology Lab, INLASA-CENETROP Ministery of Health National;
       Secretary, National Net Work of AIDS, Bolivia.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):229 (abstract no. PB0346). Unique
       Identifier : AIDSLINE ICA10/94369807
 AB    OBJECTIVE: To develop a cheap, simple and secure strategy for the
       detection of anti-HIV antibodies in saliva samples. MATERIAL AND
       METHODS: To collect the samples we used filter paper strips S.S.
       903(USA). They were placed between gums and checked for about 10 minutes
       and then dried at room temperature for 4 hours, and the samples were
       sent to the reference Laboratory. As positive control (External control)
       we used papers with saliva samples of patients who were positive for HIV
       infections with confirmatory test (LIA TEK ORGANON), also we used the
       controls of the commercial kit (Internal Controls). From the filters
       papers with sample we took 5mm diameter disk and soaked in 200-300
       microliters of working buffer solution for about 14 hours, taking 10
       microliters of this solution as samples without additional dilutions and
       working as the makers instructions (Vironostika, ORGANONN). RESULTS: We
       found 2 reactive samples in 850 studied samples by the ELISA technic, to
       confirming our findings we took serum of these patients, and used the
       confirmatory test LIA-TEK (ORGANON). CONCLUSIONS: Our findings suggest
       that it is possible to use this technique allowing developing countries
       such as Bolivia to extend the epidemiological survey in a secure and
       cheap way.
 DE    Bolivia/EPIDEMIOLOGY  Comparative Study  Enzyme-Linked Immunosorbent
       Assay  Evaluation Studies  Human  HIV Antibodies/*ANALYSIS/BLOOD  HIV
       Infections/*DIAGNOSIS/EPIDEMIOLOGY  HIV-1/*IMMUNOLOGY  Mass
       Screening/ECONOMICS/METHODS  Reagent Kits, Diagnostic
       Saliva/*IMMUNOLOGY  Salivary Proteins/*ANALYSIS  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).