Document 2773
 DOCN  M94A2773
 TI    The use of oral samples (crevicular fluid) to detect HIV-1 prevalence
       rates.
 DT    9412
 AU    Izazola-Licea JA; del Rio C; Guarner J; Lopez-Portillo M; CONASIDA
       (National AIDS Council), Mexico D.F., Mexico.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):228 (abstract no. PB0341). Unique
       Identifier : AIDSLINE ICA10/94369802
 AB    OBJECTIVE: HIV testing has been performed in different body fluids.
       Crevicular fluid (CF) is a convenient biologic fluid to collect since it
       is obtained non-invasively by rubbing the gingiva with the OraSure
       device (EpitopeINC) which renders a non infectious mucosal transudate
       rich in antibodies. Because of this advantages, we decided to test the
       use of CF as a biologic sample to detect HIV-1 antibodies compared to
       serum samples in a field study. METHODS: Paired samples of blood and CF
       were collected from: a) 369 adult men in Mexico City, selected through a
       probability survey and, b) 134 known HIV-1 positive individuals. In
       addition, 1215 CF samples were collected from the same population survey
       individuals without obtaining paired serum samples. All CF and serum
       samples were tested for HIV antibodies by ELISA (Abbott, 2nd generation
       assay). ELISA positive samples were confirmed by Western Blot
       (WB)(Organon, Tecknika). RESULTS: From the paired 369 samples of the
       population survey, ELISA was positive on 3 serum samples and 1 CF. On
       confirmatory testing using WB only the pair positive on both serum and
       CF was confirmed as a true positive. All 134 HIV-1 positive persons were
       positive on serum, CF and WB. Of the 1215 CF samples from the population
       survey 1 was positive on ELISA and confirmed by WB. We did not have any
       indeterminate results on WB. Based on the total population survey (n =
       1,584; 369 paired samples + 1215 CF samples), the HIV-1 infection rate
       among adult males in Mexico City was 0.126% (or 1.26 per thousand men;
       95% CI = 0.015-0.45%). DISCUSSION AND CONCLUSIONS: No false positive or
       false negative results were found on the CF. In contrast, there were 3
       false positive serum samples. Thus, the use of CF for HIV-1 testing for
       public health surveys seems appropriate, particularly since CF is easier
       to collect than blood by interviewers unskilled in blood collecting and
       it is not considered a high risk fluid.
 DE    Adult  Comparative Study  Enzyme-Linked Immunosorbent Assay  Evaluation
       Studies  Exudates and Transudates/*IMMUNOLOGY  False Positive Reactions
       Gingiva/*IMMUNOLOGY  Human  HIV Antibodies/*ANALYSIS/BLOOD  HIV
       Infections/DIAGNOSIS/*EPIDEMIOLOGY  HIV-1/*IMMUNOLOGY  Male
       Mexico/EPIDEMIOLOGY  Prevalence  Sensitivity and Specificity  Specimen
       Handling/INSTRUMENTATION  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).