Document 2775
 DOCN  M94A2775
 TI    Detection of antibodies to HIV-1 in saliva.
 DT    9412
 AU    Ichikawa S; Tsukano K; Ito A; Soda K; Dept. of Public Health, Yokohama
       City Univ. School of Medicine,; Japan.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):228 (abstract no. PB0340). Unique
       Identifier : AIDSLINE ICA10/94369800
 AB    OBJECTIVES: To evaluate the usefulness of saliva samples for detection
       of antibodies to HIV-1. MATERIALS & METHODS: Paired specimens of serum
       and saliva were collected from 32 HIV-1 seropositive outpatients (CDC
       type II: 25, III: 2, IV: 5) at AIDS clinic in our hospital. Saliva
       samples were collected from same seropositives periodically for 12
       months. After centrifugation, clear saliva were collected and stored at
       -40 degrees C until testing. Serum and saliva were tested with ELISA
       (ELAVIA MIXT) and western blot (WB, LAV Blot-1) method by using kits of
       Diagnostics Pasteur. RESULTS & DISCUSSION: Thirty of 32 saliva samples
       of HIV-1 seropositive cases were confirmed to be positive in ELISA test
       for anti-HIV. Two saliva samples were indeterminate, and one of them was
       collected under 4 months after the first diagnosis of HIV infection. In
       the follow-up study, samples taken from the same individuals 2 or 4
       months after first sampling showed positive results. All saliva samples
       from seropositives were constantly positive by ELISA for anti-HIV. On
       testing with the WB, 64 (71.9%) of the 89 saliva samples collected from
       28 seropositives were positive, and 25 samples were indeterminate. The
       anti GP-160 band were detected in all saliva specimens, but the
       detection rates of other bands in saliva were lower than those in serum.
       The mentioned results suggested that saliva was useful for screening of
       antibodies to HIV-1 using commercial ELISA kit.
 DE    Blotting, Western  Comparative Study  Enzyme-Linked Immunosorbent Assay
       Evaluation Studies  Human  HIV Antibodies/*ANALYSIS/BLOOD
       HIV-1/*IMMUNOLOGY  Reagent Kits, Diagnostic  Saliva/*IMMUNOLOGY
       Salivary Proteins/*ANALYSIS  Sensitivity and Specificity  MEETING
       ABSTRACT

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