Document 2830
 DOCN  M94A2830
 TI    Collection of autologous blood and bone marrow for adoptive
       immunotherapy in HIV+ve patients.
 DT    9412
 AU    Trickett A; Dwyer J; Tesfamariam N; Lam-Po-Tang R; Kwan YL; St. George
       Hospital, NSW, Australia.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):215 (abstract no. PB0288). Unique
       Identifier : AIDSLINE ICA10/94369745
 AB    OBJECTIVE: To determine the safety of harvesting large quantities of
       blood +/- bone marrow (BM) from asymptomatic HIV+ve patients. Cells were
       collected and cryopreserved for subsequent reinfusion when the patient
       progresses towards AIDS, with the aim of delaying disease onset.
       METHODS: Blood mononuclear cells were collected from 50 patients by
       leukapheresis. BM was also harvested from 6 patients. Blood samples were
       obtained pre-, post-, and one month post-procedure. Statistical analysis
       was performed using analysis of variance. RESULTS: Leukapheresis: Mean
       +/- 1SD volume collected = 334 +/- 82 mls containing 13.2 +/- 4.4 x
       10(9) nucleated cells of which 58.2 +/- 13.0% were lymphocytes. (NS =
       not significant). TABULAR DATA, SEE ABSTRACT VOLUME. CONCLUSION: Large
       volumes of blood +/- BM can be harvested safely from asymptomatic HIV+ve
       patients. Transient decreases are seen in %CD8, absolute CD4 & CD8, and
       beta 2MG following leukapheresis, which resolve within one month.
 DE    beta 2-Microglobulin/ANALYSIS  Acquired Immunodeficiency
       Syndrome/PREVENTION & CONTROL  *Blood Transfusion, Autologous  *Bone
       Marrow Transplantation  Human  HIV Core Protein p24/BLOOD  HIV
       Infections/BLOOD/*THERAPY  *Immunotherapy, Adoptive  *Leukapheresis
       Leukocyte Count  Organ Procurement  Safety  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).