Document 2947
 DOCN  M94A2947
 TI    Comparison of HIV viral burden in peripheral blood and rectal mucosa.
 DT    9412
 AU    Reka S; Kotler DP; St. Luke's-Roosevelt Hosp, New York, NY 10025.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):189 (abstract no. PB0186). Unique
       Identifier : AIDSLINE ICA10/94369628
 AB    OBJECTIVE: To compare HIV proviral DNA burden by polymerase chain
       reaction (PCR) in mononuclear cells isolated from peripheral blood (PBL)
       and rectal mucosa lamina propria (LPL), and HIV RNA by in situ
       hybridization in tissue biopsies. METHODS: 15 HIV-infected individuals
       were studied. Quantitative DNA PCR was performed on unfractionated and
       purified CD4 lymphocytes, using antibody-coated beads (Dynal Inc), using
       an LTR primer. The PCR was capable of detecting one genome copy of HIV
       DNA. HIV RNA in situ hybridization was performed using 35S labelled
       riboprobes. RESULTS: HIV DNA was detected in LPL and PBL in all
       subjects. Viral burden varied from 1 x 10(2) - 1 x 10(5) of PBL and LPL.
       Viral burden in PBL and LPL were similar in 8 cases, LPL had one log
       higher burden in 5 cases, and PBL had a higher burden in 2 cases. Cells
       hybridizing the HIV RNA probe were found in 10/14 biopsies studied. The
       results of DNA PCR did not correlate with RNA in situ results.
       CONCLUSIONS: 1) HIV proviral DNA can be amplified from both PBL and LPL,
       2) HIV viral burden of LPL and PBL may differ. 3) The viral burden (DNA)
       does not reflect virus production (RNA).
 DE    DNA, Viral/ANALYSIS  Human  HIV/*ISOLATION & PURIF  HIV
       Infections/*MICROBIOLOGY  Intestinal Mucosa/*MICROBIOLOGY  Polymerase
       Chain Reaction  Proviruses/*ISOLATION & PURIF  Rectum/*MICROBIOLOGY  T4
       Lymphocytes/MICROBIOLOGY  Viremia/*MICROBIOLOGY  Virus
       Replication/PHYSIOLOGY  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).