Document 2984
 DOCN  M94A2984
 TI    Pathogenesis of AIDS associated large cell lymphoma.
 DT    9412
 AU    Tsuzuki A; Herndier B; Marsh J; Ng V; Nolan T; Shiramizu B; McGrath M;
       University of California, San Francisco.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):18 (abstract no. 045B). Unique
       Identifier : AIDSLINE ICA10/94369591
 AB    OBJECTIVE: The pathogenesis of HIV associated Burkitt's lymphoma
       probably revolves around dysregulation of the c-myc proto-oncogene. For
       other AIDS associated lymphomas, particularly the large cell lymphomas
       (LCL), the pathogenesis is unclear, particularly when some
       pre-lymphomatous states or even frank lymphomas are polyclonal in nature
       and can in some cases lack demonstrable EBV. This study examines the
       phenotype of such lymphomas and viral and cytokine expression with the
       hopes of unravelling the key events in the early stages of
       lymphomagenesis in HIV disease. METHODS: A consecutive series of 15
       large cell lymphomas and malignant effusions were examined by the
       following techniques: 1) morphology; 2) 3-color cytofluorometry; 3)
       frozen section immunohistochemistry; 4) reverse transcriptase PCR
       followed by Southern blotting (IL-6, IL-6R, IL-10); 5) PCR, Southern
       blotting and in situ hybridization with an EBV EBER probe. RESULTS AND
       CONCLUSIONS: Twelve of 15 LCL expressed IL-6R RNA and 11/15 IL-10 RNA,
       indicating the possibility of Il-6/IL-6R and/or IL-10/IL-10R
       interactions being important in lymphoma growth. Growth studies with
       cell lines established from malignant effusions indicate both paracrine
       IL-6/IL-6R and autocrine (IL-6/IL-6R) growth analogous to what has been
       observed with human multiple myeloma. Six of 7 studied LCL were composed
       of CD5 positive B cells, the B cell thought to be important in
       autoimmunity and known to respond to IL-10 as an autocrine growth
       factor. Neutralizing antibodies to both IL-6 and IL-10 inhibited
       proliferations of two newly established AIDS LCL cell lines, suggesting
       that both IL-6 and IL-10 may play important roles in AIDS
       lymphomagenesis. Our data (7/15 cases) does not support a consistent
       role for EBV.
 DE    Antigens, CD/ANALYSIS  Blotting, Southern  Flow Cytometry  Human  HIV
       Infections/*COMPLICATIONS  Immunohistochemistry  In Situ Hybridization
       Interleukins/ANALYSIS  Lymphoma, AIDS-Related/CHEMISTRY/*GENETICS
       Lymphoma, Large-Cell/CHEMISTRY/*GENETICS  Polymerase Chain Reaction
       MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).