Document 3028
 DOCN  M94A3028
 TI    Lymphomagenesis in SIV immunosuppressed monkeys.
 DT    9412
 AU    Biberfeld P; Li SL; Kaaya EE; Feichtinger H; Rezikyan S; Putkonen P;
       Immunopathology Lab., Karolinska Institute, Stockholm, Sweden.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):17 (abstract no. 043B). Unique
       Identifier : AIDSLINE ICA10/94369547
 AB    OBJECTIVE: To study lymphomagenesis associated with SIV/HIV-2 infection
       in cynomolgus monkeys (Macaca fascicularis). METHODS: Monkeys were
       infected with SIVsm or HIV-2 and were studied for clinical signs of
       immunodeficiency and consecutive biopsies examined for B-cell clones and
       association with herpes virus, by immunohistopathology, PCR, Southern
       blot (SB), in situ hybridization, electron microscopy and cell culture.
       RESULTS, DISCUSSION AND CONCLUSIONS. Monkeys infected with SIVsm but not
       these with HIV-2 developed frequently (21/51) malignant lymphomas.
       Lymphoma induction time varied from 5 to 46 months. All monkeys with
       lymphoma were markedly immunosuppressed. Most lymphomas were primarily
       extranodal including CNS, of high-grade malignant histology, of B-cell
       origin and associated with a B-lymphotropic herpes virus related to
       human EBV (HVMF). HVMF showed considerable restriction map homology with
       EBV in the EBNA-1,2 regions and within the IR1, the EBER and the ori-P
       regions, but not with LMP regions. HVMF from a monkey lymphoma cell line
       transformed in vitro normal monkey B-cells. By in situ hybridization for
       EBER-RNA, HMVF associated lymphoma cells could be detected before
       clinical lymphoma manifestation. This was also indicated by the early
       appearance of a dominant clone according to DNA-PAGE of the VDJ
       immunoglobulin heavy chain region. These observations indicate a marked
       similarity of lymphomas in SIV immunosuppressed monkeys with human
       AIDS-lymphomas associated with EBV and the possibility to use this
       monkey lymphoma model for evaluation of therapeutic protocols.
 DE    Animal  Disease Models, Animal  Herpesvirus 4, Human/GENETICS  *HIV-2
       Immunosuppression  Lymphoma, AIDS-Related/*COMPLICATIONS/MICROBIOLOGY
       Macaca fascicularis/IMMUNOLOGY  Restriction Mapping  Simian Acquired
       Immunodeficiency Syndrome/*COMPLICATIONS/  MICROBIOLOGY  *SIV/GENETICS
       Time Factors  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).