Document 3032
 DOCN  M94A3032
 TI    Vesnarinone inhibits AIDS-KS cells in culture.
 DT    9412
 AU    Petit RG; Miles S; Magpantay L; Mitsuyasu R; Otsuka America
       Pharmaceutical, Rockville, MD.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):169 (abstract no. PB0104). Unique
       Identifier : AIDSLINE ICA10/94369543
 AB    OBJECTIVE: Vesnarinone, OPC8212, is an inhibitor of TNF and IL-6
       production in some cell culture systems. Our group has previously shown
       that these factors act as mitogens for AIDS-KS in culture. We wanted to
       determine if vesnarinone inhibits AIDS-KS cells in culture. METHODS:
       Cultured AIDS-KS cells were plated at 2,000 cells/well in 24 well plates
       and allowed to adhere overnight. Vesnarinone was dissolved in 100% DMSO
       and added to fresh media and cells at concentrations from 10(-8) to
       10(-5) M. Controls included serum free media with ITS+, 100% ethanol,
       100% DMSO, dexamethasone in DMSO and retinal in DMSO at similar
       concentrations. Cell counts were performed on days 3 and 6 using a
       Coulter model ZM particle counter. The assays were repeated 4 times with
       three different cell lines. RESULTS: Vesnarinone reproducibly inhibited
       AIDS-KS cell proliferation at the 10(-6) to 10(-5) M range. Allow though
       there was a clear solvent effect, the inhibition was seen regardless of
       the conditions used (serum free, etc.) thus demonstrating a true drug
       effect. Although there is superior inhibition at higher drug levels,
       these are not achievable in patients. CONCLUSIONS: Vesnarinone inhibits
       AIDS-KS cells in culture at pharmacologically achievable concentrations.
       This agent might be useful in the clinical management of AIDS-KS.
 DE    Acquired Immunodeficiency Syndrome/*PATHOLOGY  Antiviral
       Agents/*PHARMACOLOGY  Cell Division/*DRUG EFFECTS  Culture Media
       Dose-Response Relationship, Drug  Human  Interleukin-6/ANTAGONISTS &
       INHIB  Quinolines/*PHARMACOLOGY  Sarcoma, Kaposi's/*PATHOLOGY  Skin
       Neoplasms/*PATHOLOGY  Tumor Cells, Cultured/*DRUG EFFECTS  Tumor
       Necrosis Factor/ANTAGONISTS & INHIB  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).