Document 3038
 DOCN  M94A3038
 TI    Correlation of selected CD8 lymphocyte activation markers with
       quantitative cellular viremia in asymptomatic HIV infected individuals.
 DT    9412
 AU    Levacher-Clergeot M; Bouscarat F; Dazza MC; Sinet M; Strauss K; Girard
       PM; Rozenbaum W; Coulaud JP; Pocidalo JJ; INSERM U13, Paris, France.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):168 (abstract no. PB0100). Unique
       Identifier : AIDSLINE ICA10/94369537
 AB    OBJECTIVE: Multi-colour flow cytometry has demonstrated significant
       alterations within the CD8 subsets co-expressing activation markers in
       HIV infection. Our study focuses on the correlation of these CD8 subsets
       with viral load in asymptomatic HIV infected patients with no marked CD4
       lymphocyte depletion. PATIENTS AND METHODS: Blood samples were obtained
       from 49 HIV infected patients and 23 HIV- individuals. All HIV infected
       patients were asymptomatic, were accrued within two years of diagnosis
       (14.4 +/- 1.3 months), and had a CD4 cell count above 400
       cells/microliters (788 +/- 43). An extensive panel of three colour
       reagent combinations was assessed by flow cytometry. Levels of HIV
       viremia were measured both in PBMC's and in plasma using end point
       dilution cultures. RESULTS: Thirty-nine patients had a positive cellular
       viremia (0.5 to 5315 TCID50/10(6) cells), while only 5 had a positive
       plasma viremia. Mean CD8% was 50.9 +/- 1.5 in patients versus 33.3 +/-
       1.3 in controls. Bright CD8 accounted for the expansion of the CD8
       cells. All subset results are in proportion of the bright CD8
       population. Table: mean +/- SE of relevant values and respective
       correlation coefficient-r versus cellular viremia. TABULAR DATA, SEE
       ABSTRACT VOLUME. CONCLUSIONS: We confirm and extend the finding of
       increased specific CD8 subsets to asymptomatic HIV infected individuals
       with no marked CD4 depletion. Among these subsets, %CD38+CD45RO+ and
       %CD38+CD28- are significantly correlated to cellular viremia. HIV
       plasmatic RNA quantification is in progress. A longitudinal study will
       determine if these subsets can be used as a prognostic indicator.
 DE    Antigens, CD28/BLOOD  Antigens, CD8/*BLOOD  Antigens,
       Differentiation/BLOOD  Flow Cytometry  Follow-Up Studies  Human
       HIV/*IMMUNOLOGY  HIV Infections/DIAGNOSIS/*IMMUNOLOGY  HLA-DR
       Antigens/BLOOD  Leukocyte Count  Lymphocyte Transformation/*IMMUNOLOGY
       Monocytes/IMMUNOLOGY/MICROBIOLOGY  T-Lymphocytes,
       Suppressor-Effector/*IMMUNOLOGY  T4 Lymphocytes/IMMUNOLOGY
       Viremia/DIAGNOSIS/*IMMUNOLOGY  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).