Document 3040
 DOCN  M94A3040
 TI    Viral phenotype and clinical outcome in HIV+ children.
 DT    9412
 AU    Munoz-Fernandez MA; Navarro J; Obregon E; Garcia D; Gurbindo D;
       Sampelayo T; Fernandez-Cruz E; Dept. Immunology, H. Gregorio Maranon,
       Madrid, Spain.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):167 (abstract no. PB0095). Unique
       Identifier : AIDSLINE ICA10/94369535
 AB    OBJECTIVE: To investigate whether highly replicative HIV-1 isolates in
       children has the ability to induce syncytium formation (SI) in PBMC and
       MT-2 cells, whereas slow/low viruses lack this capacity. METHODS:
       Sixty-four infants born of seropositive HIV mother were studied. Virus
       isolation was performed by cocultivation of the patient's PBMC cells
       with donor lymphocytes. Viral phenotype: PHA-stimulated PBMC were
       infected with cell-free supernatants from the virus cocultures (VC).
       After 7-10 days the infected PBMC were cultured with MT-2 cells and HIV
       were characterized for SI, and p24 Ag was quantified sequentially in VC
       supernatants. RESULTS: Only 14 out of 64 (22%) infants were positive by
       PCR and VC. HIV isolates were available in 13 out of 14 infected
       children. Of these 13 isolates, 6 were classified as rapid/high and 7 as
       slow/low virus. Highly replicative HIV isolates induced syncytia in PBMC
       and MT-2 cells, whereas slow/low virus did not. DISCUSSION AND
       CONCLUSIONS: Infants with rapid/high isolates will have a more rapid
       progressive course during the first months of life and higher levels of
       p24 antigenemia, while infants with slowly replicating HIV will do
       better.
 DE    Giant Cells/MICROBIOLOGY  Human  HIV Core Protein p24/BLOOD  HIV
       Seropositivity/CLASSIFICATION/*DIAGNOSIS/MICROBIOLOGY
       HIV-1/*PATHOGENICITY  Infant  Infant, Newborn  Prognosis  Slow Virus
       Diseases/CLASSIFICATION/DIAGNOSIS/MICROBIOLOGY  Virulence  Virus
       Replication/*PHYSIOLOGY  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).