Document 3044
 DOCN  M94A3044
 TI    Two-color flow cytometric determination of absolute counts of lymphocyte
       subpopulations in HIV-infected patients.
 DT    9412
 AU    Goto T; Takano K; Kimura S; Shionogi Biomed. Lab., Univ. of Tokyo,
       Japan.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):166 (abstract no. PB0090). Unique
       Identifier : AIDSLINE ICA10/94369531
 AB    OBJECTIVE: To find out good surrogate markers for analysis of
       pathophysiology and prediction of prognosis of HIV disease. SUBJECTS AND
       METHODS: Absolute counts of lymphocyte subpopulations (CD4 x CD45RA, CD4
       x CD29, CD8 x CD11b, CD16 x CD56) were analyzed by two-color
       flowcytometry using Cytron-Absolute (Ortho) in 74 patients with HIV
       infection (CDC category A, B, C). In 8 patients, time courses of
       absolute counts of subpopulations were evaluated. Half of the patients
       were hemophiliacs. RESULTS: 1) CD4 counts measured by Cytron-Absolute
       seemed to be more reliable than those obtained through conventional
       method by calculation as WBC x lymphocyte (%) x CD4 (%). 2) Absolute
       counts of all of CD4+ subpopulations (CD4+CD45RA+, CD4+CD45RA-,
       CD4+CD29+, CD4+CD29-) declined almost parallel during the course of the
       desease. 3) Absolute counts of CD8+CD11b- (Tc) declined more slowly than
       CD4+ counts did. Opportunistic infections (OIs) frequently observed when
       CD8+CD11b- counts declined to a level below 200 cells/microliters. Thus,
       CD8+CD11b- counts may be a better surrogate marker for probability of
       OIs than CD4+ counts. 4) Absolute counts of CD16+CD56- NK cells stayed
       relatively constant when compared with CD16+CD56+ NK cells which
       declined steadily with time. CONCLUSIONS: Preliminary study on absolute
       counts of lymphocyte subpopulations was carried out. Some of the
       subpopulations seemed to be useful for the analyses of pathophysiology
       and prognosis of HIV disease.
 DE    AIDS-Related Opportunistic Infections/CLASSIFICATION/DIAGNOSIS/
       IMMUNOLOGY  Biological Markers  Flow Cytometry/*METHODS
       Hemophilia/IMMUNOLOGY  Human  HIV
       Infections/CLASSIFICATION/DIAGNOSIS/*IMMUNOLOGY  Killer Cells,
       Natural/IMMUNOLOGY  Leukocyte Count  Prognosis  T-Lymphocyte
       Subsets/*IMMUNOLOGY  T4 Lymphocytes/IMMUNOLOGY  MEETING ABSTRACT

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