Document 3046
 DOCN  M94A3046
 TI    Heat versus acid dissociation of immune complexes for the detection of
       P24 antigenemia.
 DT    9412
 AU    Schechter M; Oliveira E; Pinto ME; Souza VM; Bronstein M; AIDS Program,
       Federal University of Rio de Janeiro, Bronstein; Lab., Brazil.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):166 (abstract no. PB0089). Unique
       Identifier : AIDSLINE ICA10/94369529
 AB    INTRODUCTION: P24 antigenemia is widely used as a surrogate marker of
       disease progression and of therapeutic efficacy. Immune complex
       dissociation has been shown to greatly increase the sensitivity of p24
       detection assays and has become the standard technique. OBJECTIVE: To
       compare heat (HD) versus acid (AD) dissociation of immune complexes in
       p24 antigen capture assays. METHODS: Sera from HIV infected patients
       previously known to be p24 negative by AD were assayed in parallel after
       AD or HD, using the Coulter antigen kit. AD was performed using glycine
       buffer followed by neutralization with Tris buffer. HD was accomplished
       by diluting sera 1:3 in distilled water, followed by immersion of the
       tubes in a boiling water bath for 5 minutes. The level of p24
       antigenemia was quantified by comparing the optical density of reactive
       samples to a standard curve of known positive specimens ran on the same
       assay. RESULTS: Of the 39 samples tested, all remained negative by AD
       and 3 (7.7%; .95 CI = 0-16%) became positive after HD. CONCLUSION: HD of
       immune complexes is cheaper, easier to perform and far less
       labour-intensive than AD. In addition, it may increase the sensitivity
       of p24 antigen detection assays.
 DE    Antigen-Antibody Complex/*BLOOD  AIDS Serodiagnosis/*METHODS
       Comparative Study  Human  HIV Core Protein p24/*BLOOD  HIV
       Infections/*DIAGNOSIS/IMMUNOLOGY  Predictive Value of Tests  Support,
       Non-U.S. Gov't  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).