Document 3053
 DOCN  M94A3053
 TI    Precision and stability of CD4, 8 and 3 values in HIV+ and
       HIV-populations in Shirati Tanzania as evaluated by FACSCount.
 DT    9412
 AU    Strauss K; Brubaker G; Kumbi D; Shao J; Krenkler W; Blattner W; Levin A;
       Becton Dickinson, Europe, Belgium.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):164 (abstract no. PB0084). Unique
       Identifier : AIDSLINE ICA10/94369522
 AB    The performance characteristics of a dedicated CD4 counting system,
       FACSCount, were evaluated in populations from Shirati, Tanzania.
       Precision assessments involved 4 replicate stainings and analyses per
       subject. The pooled CV's of the parameters evaluated (CD4,8,3 and 4:8
       ratio) are presented below: TABULAR DATA, SEE ABSTRACT VOLUME. Stability
       assessments involved 4 replicate analyses per subject, at time points 0,
       24, 48 and 72 hours, of both unstained aliquots and aliquots stained at
       0 hour. Aged blood testing revealed no significant differences between
       any time points. ANOVA aged stain testing revealed the following
       differences (p < 0.05): TABULAR DATA, SEE ABSTRACT VOLUME. CONCLUSION:
       FACSCount provides precise and stable stained and unstained results in
       the large majority of HIV- and HIV+ Tanzanian subjects tested.
 DE    Antigens, CD3/*BLOOD  Antigens, CD4/*BLOOD  Antigens, CD8/*BLOOD
       CD4-CD8 Ratio  *Developing Countries  Human  HIV
       Seronegativity/*IMMUNOLOGY  HIV
       Seropositivity/CLASSIFICATION/DIAGNOSIS/*IMMUNOLOGY  Leukocyte Count
       Reference Values  Reproducibility of Results  T-Lymphocyte
       Subsets/*IMMUNOLOGY  Tanzania  T4 Lymphocytes/IMMUNOLOGY  MEETING
       ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).