Document 3060
 DOCN  M94A3060
 TI    Monitoring of CD8/DR lymphocyte subset in pediatric HIV infection: a
       preliminary study.
 DT    9412
 AU    Tzantzoglou S; Ajassa C; Berardelli G; Falciano M; Bellagamba R; Catania
       S; Infect. Dis. La Sapienza Univ., Rome, Italy.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):162 (abstract no. PB0075). Unique
       Identifier : AIDSLINE ICA10/94369515
 AB    OBJECTIVE: to value the presence of quantitative changes of CD8/DR in
       different stages of pediatric HIV infection and their significance as a
       predictive marker of disease progression. METHODS: we followed the
       course of CD4, CD8, CD8/DR, beta 2M, HIV-Ag in 6 children with HIV
       infection by vertical trasmission (mean age: 6 years) in different
       stages of infection: 4 patients (3 P2D1,1P2BD1) followed for a period of
       8 months and 2 patients (1 P2A;1 P2F) for 3 months. The HIV-negative
       control group consisted in 8 children (4 healthy subjects and 4 with
       acute viral infections). Fresh peripheral venous blood was obtained from
       each child in EDTA tubes. Three-colour lymphocyte immunophenotyping was
       performed using PerCP-conjugated anti-CD4 monoclonal antibody in
       combination with anti-CD8(FICT) and anti-HLA-DR (PE) monoclonal
       antibodies. The resulting immunostained white blood cells were analyzed
       on a FACScan, using the PAINT A GATE program. The lymphocyte gate was
       defined on the scattergram. The presence of HIV-Ag and beta 2M in serum
       was determined by ELISA. RESULTS: in all HIV patients we observed a
       progressive decrease of CD4 and in 5 cases also an increase of CD8.
       Three patients (P2A,P2F,P2BD1) showed a percentage of CD8/DR lower than
       50% associated with low levels of beta 2M and absence of HIV-Ag in
       serum. Three patients (P2D1) presented a percentage of CD8/DR higher
       than 50% associated with high levels of beta 2M. The highest values of
       CD8/DR were observed during viral acute infections (HHV6-CMV-EBV).
       HIV-Ag was present in the serum of 1 patient for the whole period of
       study. In another patient, HIV-Ag showed a progressive decrease until
       the complete depletion, associated with a drop of CD8/DR. The percentage
       of CD8/DR was 0-4% in healthy children and lower than 50% in
       HIV-negative subjects with acute viral infections. DISCUSSION: Our
       preliminary studies show that also in the course of pediatric HIV
       infection quantitative changes of CD8/DR occurr. High levels of CD8/DR
       were observed mainly in association with acute viral infections
       (CMV-EBV-HHV6). The increase of CD8/DR seems to be correlated with
       elevated values of beta 2M, but not always with serum HIV-Ag. However,
       the monitoring of CD8/DR together with the classic surrogate markers
       needs further prospective follow-up studies in order to ascertain the
       immunopathogenetic and prognostic significance of this cellular subset
       in pediatric HIV infection.
 DE    beta 2-Microglobulin/METABOLISM  Antigens, CD4/BLOOD  Antigens,
       CD8/*BLOOD  Biological Markers/BLOOD  Child  Female  Follow-Up Studies
       Human  HIV Antigens/BLOOD  HIV
       Infections/CLASSIFICATION/*DIAGNOSIS/IMMUNOLOGY  HLA-DR Antigens/*BLOOD
       Immunophenotyping  Leukocyte Count  Male  T-Lymphocyte
       Subsets/*IMMUNOLOGY  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).