Document 3064
 DOCN  M94A3064
 TI    CD4+/CD8+ subset immunoassay in HIV+ adults and children.
 DT    9412
 AU    Denny T; Jensen B; Garcia A; Vella F; Gavin E; Stein D; Oleske J; Wong
       W; UMDNJ, Newark.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):161 (abstract no. PB0071). Unique
       Identifier : AIDSLINE ICA10/94369511
 AB    OBJECTIVE: The purpose of this study was to compare the absolute CD4+
       and CD8+ lymphocyte levels obtained by the predicate flow
       cytometry/hematology method with those resulting from a new alternative
       fluorescent immunoassay (Zymmune CD4/CD8 Cell Monitoring Kit, Zynaxis,
       Inc., Malvern, PA) in both adult and pediatric specimens. METHODS: In
       this study, 495 samples (75% adult and 25% pediatric) of EDTA
       anticoagulated peripheral blood were submitted for routine lymphocyte
       immunophenotyping as part of their clinical follow up. All samples were
       processed within 6 hours of collection. Absolute lymphocyte counts and
       relative CD4+ and CD8+ lymphocyte levels were assessed using
       standardized protocols on a Sysmex E-2500 hematology instrument and a
       FACScan cytometer, respectively. RESULTS: A high degree of correlation
       was found between the predicate and Zymmune methods for both adult and
       pediatric specimens. Correlation coefficients for adults were 0.927
       (CD4) and 0.883 (CD8), with pediatrics values of 0.912 (CD4) and 0.927
       (CD8). CONCLUSIONS: The Zymmune and predicate methods provide
       substantially equivalent measurements of the CD4+ and CD8+ lymphocyte
       counts in whole blood specimens.
 DE    Adult  Child  Comparative Study  CD4-CD8 Ratio  Flow Cytometry
       Fluoroimmunoassay  Human  HIV Seropositivity/DIAGNOSIS/*IMMUNOLOGY
       Leukocyte Count  Predictive Value of Tests  MEETING ABSTRACT

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