Document 3068
 DOCN  M94A3068
 TI    Variant HIV RNA distribution within lymph nodes and tonsils of stage
       matched patients.
 DT    9412
 AU    Turnicky R; Anderson D; Nau M; Zhao F; Coats J; Mayers D; Division of
       Retrovirology, Walter Reed Army Institute of; Research, Rockville, MD
       20850.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):160 (abstract no. PB0067). Unique
       Identifier : AIDSLINE ICA10/94369507
 AB    OBJECTIVE: We used human lymphoid tissue as a model to better define
       early HIV staging and possibly monitor response to therapies. METHODS: 5
       Lymph node and 3 tonsil biopsies were obtained from stage II matched
       patients, and 1 non-HIV control. Tissues were formalin fixed, and snap
       frozen in liquid nitrogen. Immunoperoxidase staining was performed (CD4,
       CD8, CD35 and IgD), followed by in-situ hybridization (ISH) using a full
       lengh sheared HIV-1 probe. RESULTS: ISH showed intense positivity
       localized to germinal centers (GC). Differences in HIV distribution were
       apparent between stage matched nodes. The nodes with advanced
       involutional features showed greater numbers of individual cells
       expressing HIV RNA in both the GC and interfollicular area (IFA). This
       is contrasted to a diffuse pattern of distribution, with less individual
       cell positivity, in nodes lacking involutional changes. DISCUSSION: GCs
       serve as repositories for HIV. It is apparent that there is altered
       viral distribution in lymphoid tissue in early stage disease amongst
       stage matched patients. Altered distribution correlated with tissue
       morphology in that tissues with follicular involtion had a greater
       number of individually positive cells both within the GC and IFA. These
       were identified as CD4 positive cells, contrasting to very few cells
       positive and a DRC related pattern in those tissues with absent
       involution. Those with a DRC pattern, may have less advanced disease as
       evidenced by the lack of GC involution and a more intact immune system.
       Tissue biopsy serve as a method to augment patient staging in early HIV
       disease and serve as a means to monitor therapy.
 DE    Gene Expression Regulation, Viral/PHYSIOLOGY  Human  HIV
       Infections/CLASSIFICATION/*PATHOLOGY  HIV-1/*GENETICS  Immunoenzyme
       Techniques  Lymph Nodes/*PATHOLOGY  RNA, Viral/*GENETICS
       Tonsil/*PATHOLOGY  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).