Document 3084
 DOCN  M94A3084
 TI    Structural change in oligosaccharide chains of IgG in HIV-1 infected
       individuals.
 DT    9412
 AU    Mizuochi T; Nakata M; Oka S; Shimada K; School of Engineering, Tokai
       University, Kanagawa, Japan.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):157 (abstract no. PB0054). Unique
       Identifier : AIDSLINE ICA10/94369491
 AB    OBJECTIVE: To determine if structures of oligosaccharide chains of IgG
       from HIV-1 infected individuals change according to the disease
       development. METHODS: IgG was purified from sera of hemophiliacs (n = 5)
       and HIV-1 infected hemophiliacs at CDC stage 2 (n = 5), 3 (n = 5), and 4
       (n = 4) by ammonium sulfate precipitation followed by column
       chromatography with DEAE-cellulose and Sephacryl S-300. The
       oligosaccharide chains were liberated from IgG by hydrazinolysis and
       labelled with p-aminobenzoic acid ethyl ester, and the structures were
       analyzed by enzymatic microsequencing and HPLC. RESULTS: Although no
       difference from the uninfected individuals in the structures of IgG
       oligosaccharides was observed in CDC2, the level of galactosylation of
       the IgG oligosaccharide chains was higher at CDC3 than CDC2 (P < 0.03)
       and lower at CDC4 than CDC3 (P < 0.002). CONCLUSION: The oligosaccharide
       chains of serum IgG could be a useful biochemical marker for the
       follow-up of HIV-1 infected individuals. The structural change suggested
       the involvement of subpopulation of B cells in the process of the
       disease development.
 DE    B-Lymphocyte Subsets/IMMUNOLOGY  Chromatography, High Pressure Liquid
       Human  HIV Infections/CLASSIFICATION/*DIAGNOSIS/IMMUNOLOGY
       HIV-1/*IMMUNOLOGY  IgG/BLOOD/*CHEMISTRY
       Oligosaccharides/BLOOD/*CHEMISTRY  MEETING ABSTRACT

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