Document 3133
 DOCN  M94A3133
 TI    Envelope V2 and V3 regions in circulating HIV-1 from patients with
       SI-variants.
 DT    9412
 AU    Telenti A; Grunow R; Deiss V; Bernasconi C; Matter L; von Overbeck J;
       Univ. of Berne, Switzerland.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):146 (abstract no. PB0009). Unique
       Identifier : AIDSLINE ICA10/94369442
 AB    BACKGROUND: SI phenotype may result from selection of a minority
       species. To evaluate this possibility, we studied match serum and MT-2
       culture supernatant. The V2 and V3 of gp120, known determinants of a SI
       phenotype, were sequenced to establish how accurately cultured SI
       isolates reflect the circulating species in vivo. M&M: Samples from 14
       patients were evaluated by culture in MT-2 cells, and sequencing of env
       from multiple clones obtained from culture supernatant and corresponding
       sera. A SI genotype was defined as presence of elongation of the V2
       hypervariable region and/or increase in positive charge of the V3 loop.
       RESULTS: five groups were defined: TABULAR DATA, SEE ABSTRACT VOLUME.
       CONCLUSIONS: 1) V2 and/or V3 SI-genotype was always present in culture
       isolates exhibiting a SI phenotype. 2) The characteristics of V2 in
       culture--but not V3--reflected what was found in serum in most patients.
       3) Only 3 patients exhibited a fully homogeneous population of
       SI-genotype variants in vivo and in vitro. Whether these patients
       experience a different clinical evolution, and the possibility that MT-2
       assays not only select minority strains, but allow the outgrowth of
       variants not present in vivo need further assessment.
 DE    Acquired Immunodeficiency Syndrome/*MICROBIOLOGY  Cell Line  Gene
       Products, env/BIOSYNTHESIS/*GENETICS  *Genes, env  Genotype  Human
       HIV-1/*GENETICS/*ISOLATION & PURIF  Phenotype  Variation (Genetics)
       MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).