Document 3138
 DOCN  M94A3138
 TI    Seronegativation in an asymptomatic HIV1 infected patient.
 DT    9412
 AU    Sousa A; Lourenco MH; Ferreira MO; Teles LC; Victorino RM; Faculdade de
       Medicina, Lisboa, Portugal.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):145 (abstract no. PB0008). Unique
       Identifier : AIDSLINE ICA10/94369437
 AB    OBJECTIVE: To report immunological and virological studies in an
       asymptomatic HIV infected patient in wham seronegativation was
       documented. METHODS: Two different ELISA methods were done as initial
       tests for HIV1 antibodies followed by Western blot to identify
       individual proteins sequentially done during the 6 years follow-up
       period. Flow cytometry was used for quantitation of CD3+, CD8+, CD45RO+,
       CD45RA+, cells in peripheral blood. Lymphocyte proliferative responses
       to mitogens was measured by 3H thymidine incorporation in peripheral
       blood mononuclear cells cultured in triplicate. Similarly responses to
       HIV1 and HIV2 recombinant proteins in three different concentrations (gp
       120, gp 105, p 24 and p 26 provided by the MRC-U.K.) were studied using
       24 replicates (24 culture wells for each concentration of protein).
       RESULTS: The patient was a 48 year old female who was tested for HIV in
       a blood bank and was found to have a clearly positive ELISA test and
       antibodies to all HIV1 strutural proteins on two different occasions (gp
       160, gp 110, gp 41, p 24, p 18). Although the patient lived in Portugal,
       her sexual partner had lived long periods in Central Africa. She was
       referred to an HIV clinic where she was followed for 6 years. A
       progressive loss of antibodies to the different proteins was recorded in
       the sequential Western blots (first to envelope proteins and finally to
       core). She has presently no antibodies to any of the HIV proteins.
       Levels of CD4+, CD8+, CD45RO+, CD45RA+ cells were normal along the
       follow-up. Studies on the T helper responses to recombinant HIV1
       proteins (core and envelope) will be presented. CONCLUSION: This case
       illustrates the possibility of seronegativation in HIV1 infection and
       this will be discussed considering the epidemiological data and the
       results of the immunological studies.
 DE    Case Report  Enzyme-Linked Immunosorbent Assay/METHODS  Female  Flow
       Cytometry  Human  HIV Antibodies/*BLOOD  HIV Seronegativity/*IMMUNOLOGY
       HIV Seropositivity/*IMMUNOLOGY  *HIV-1  Lymphocyte Transformation
       Lymphocytes/IMMUNOLOGY  Middle Age  T-Lymphocytes,
       Helper-Inducer/IMMUNOLOGY  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).