Document 3145
 DOCN  M94A3145
 TI    Replication of Chlamydia trachomatis and Chlamydia pneumoniae in human
       monocytic cell line U-937.
 DT    9412
 AU    Numazaki K; Suzuki K; Chiba S; Department of Pediatrics, School of
       Medicine, Sapporo Medical; University, Japan.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):142 (abstract no. PA0187). Unique
       Identifier : AIDSLINE ICA10/94369430
 AB    OBJECTIVE: Despite the various pathogenic effects of Chlamydiae, there
       is only limited direct evidence that chlamydia infections occur in
       monocytes and macrophages. The present study was designed to determine
       the effects of C. trachomatis and C. pneumoniae on monocytes-macrophages
       and the mechanisms of persistence in these cells. METHODS: For the
       purpose of infection, U-937 cells were pretreated with polybrene and
       inoculated with C. trachomatis L2 or C. pneumoniae TW-183. Cells and
       media were harvested at intervals of 72 h. Polymerase chain reaction
       (PCR) and endonuclease analysis were used to detect chlamydial genomes
       from harvested cells. The expression of surface antigens on
       Chlamydia-infected U-937 cells was assessed by flow cytometry (FCM).
       RESULTS: Chlamydial DNA which code ompA of major outer membrane protein
       were detected from C. trachomatis L2-inoculated U-937 cells over a
       period of 150 days by PCR. Chlamydial DNA had been detected also from C.
       pneumoniae TW-183-inoculated U-937 cells. U-937 cells infected with C.
       pneumoniae expressed higher level of Leu-M1 by FCM. DISCUSSION AND
       CONCLUSIONS: It is likely that monocytes-macrophages play an important
       role in the persistence of infections of C. trachomatis or C. pneumoniae
       and act as reservoirs for dissemination.
 DE    Cell Line  Chlamydia pneumoniae/GENETICS/*PHYSIOLOGY  Chlamydia
       trachomatis/GENETICS/*PHYSIOLOGY  *DNA Replication  DNA,
       Bacterial/*BIOSYNTHESIS  Flow Cytometry/METHODS  Human  Lymphoma,
       Large-Cell  Macrophages/*MICROBIOLOGY  Monocytes/*MICROBIOLOGY
       Polymerase Chain Reaction/METHODS  Restriction Mapping  Tumor Cells,
       Cultured  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).