Document 3150
 DOCN  M94A3150
 TI    Inhibition of human herpesvirus 7 infection by anti-CD4 monoclonal
       antibodies and HIV-1 gp120.
 DT    9412
 AU    Yasukawa M; Yakushijin Y; Furukawa M; Hato T; Takada K; Fujita S; First
       Dep. Int. Med., Ehime University School of Medicine, Japan.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):141 (abstract no. PA0184). Unique
       Identifier : AIDSLINE ICA10/94369425
 AB    OBJECTIVE: We have recently found that human herpesvirus (HHV)-7 shows
       tropism to CD4+ T cells, but not to CD4- cells, and that the transport
       of intracytoplasmic CD4 molecules to the cell surface is impaired in
       HHV-7-infected cells (J. Immunol. in press). Based on these findings, we
       speculated that the CD4 molecule might be the receptor for HHV-7, and
       the present study was performed to verify this possibility. METHODS: The
       ability of anti-CD4 V1 domain monoclonal antibodies (mAbs) to inhibit
       the replication of HHV-7 was examined by detection of cytopathic effect,
       indirect immunofluorescence, 50% tissue culture infection dose and
       quantitative PCR for the virus genome. Similarly, the effect of HIV-1
       gp120 on the replication of HHV-7 was examined. RESULTS: Anti-CD4 V1
       domain mAbs, which inhibit HIV-1 infection, also appeared to inhibit the
       replication of HHV-7. None of anti-CD4 mAbs affected the replication of
       HHV-6. Pretreatment of CD4+ T cells with recombinant HIV-1 gp120 also
       resulted in inhibition of HHV-7 infection. DISCUSSION AND CONCLUSIONS:
       These data strongly suggest that the CD4 molecule is the major receptor
       for HHV-7, and that HIV-1 and HHV-7 compete for CD4 as their binding
       receptor. The identification of the HHV-7 structure binding to CD4
       molecule would shed light on the development of the subcomponent peptide
       derived from HHV-7, which selectively inhibits HIV binding but not
       affect CD4+ T-cell functions.
 DE    Antibodies, Monoclonal/*PHARMACOLOGY  Antigens,
       CD4/IMMUNOLOGY/*PHYSIOLOGY  Comparative Study  Herpesvirus 7, Human/DRUG
       EFFECTS/*PHYSIOLOGY/PATHOGENICITY  Human  HIV Envelope Protein
       gp120/*PHARMACOLOGY  HIV-1/*PHYSIOLOGY  Receptors, Virus/DRUG
       EFFECTS/PHYSIOLOGY  Recombinant Proteins/*PHARMACOLOGY
       T-Lymphocytes/DRUG EFFECTS/IMMUNOLOGY/*MICROBIOLOGY  T4 Lymphocytes/DRUG
       EFFECTS/IMMUNOLOGY/*MICROBIOLOGY  Virus Replication/*DRUG
       EFFECTS/IMMUNOLOGY  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).