Document 3152
 DOCN  M94A3152
 TI    Comparison of human herpesvirus-6 (HHV-6) variant A and B genes.
 DT    9412
 AU    Yamanishi K; Yamamoto T; Isegawa Y; Mukai T; Osaka University, Japan.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):141 (abstract no. PA0182). Unique
       Identifier : AIDSLINE ICA10/94369423
 AB    OBJECTIVE: HHV-6 is now classifird into two variants such as HHV-6A and
       HHV-6B. The gene product of putative immediate-early(IE) loci in HHV-6
       variant A was reported to transactivate human immunodeficiency virus
       (HIV). Here we report the comparison of sequences in strain HST, variant
       B isolated from a Japanese patient with roseola infantum with those of
       HHV-6A. METHODS: The virus used in this study was the HST strain and a
       Pst I library was constructed using plasmid pUC19 and genomic DNA. Both
       strands of DNA were sequenced by the dideoxynucleotide chain termination
       method and the sequence of this virus was compared with those of variant
       A which was already published(Martin et al., 1991). RESULTS AND
       DISCUSSION: Approximately 70% of total DNA in HHV-6B was sequened,
       including regions of IE loci. A computer search of one of the IE loci
       uncovered a similarity to the IE locus of HHV-6 strain U1102 (J Virol,
       1993). Both sequences were highly conserved except occasional base
       changes, but U1102 had deletions of 108 and 228 base pairs within the
       ORF. Furthermore, the HST ORF was 65 codons shorter than the ORF of
       U1102 at the 3' end. The HST and U1102 ORFs shared approximately 85% DNA
       homology and 70% amino acid homology. Although it was reported that the
       product of this region in HHV-6A transactivated LTR of HIV, no or less
       activity was found in HHV-6B. The comparison of DNA sequences in both
       variants and the transactivation activity to HIV in other regions will
       be discussed.
 DE    Amino Acid Sequence  Base Composition  Base Sequence  Comparative Study
       Conserved Sequence  DNA, Viral/*GENETICS/ISOLATION & PURIF  *Genes,
       Immediate-Early  Herpesvirus 6, Human/*GENETICS  HIV/*GENETICS  *HIV
       Long Terminal Repeat  Open Reading Frames  Sequence Deletion  Sequence
       Homology, Amino Acid  Sequence Homology, Nucleic Acid  Software
       Trans-Activation (Genetics)  *Variation (Genetics)  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).