Document 3155
 DOCN  M94A3155
 TI    Lipids of Mycoplasma fermentans have an activity of HIV induction.
 DT    9412
 AU    Matsuda K; Sanuma K; Taki T; Handa S; Yamamoto N; Harasawa R; Tokyo Med.
       and Dent. Univ., Japan.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):140 (abstract no. PA0180). Unique
       Identifier : AIDSLINE ICA10/94369420
 AB    OBJECTIVE: Mycoplasma fermentans is suspected as a cofactor of AIDS, but
       the mechanism of pathogenesis is not well known. We purified and
       characterized novel phosphorylcholine-containing glycoglycerolipid from
       HIV- and HTLV-I-infected human helper T-cell culture, and the lipids
       were found to be from accidentally infected Mycoplasma fermentans. To
       elucidate the role of the lipids, the activity of HIV induction from
       latently HIV-infected macrophage cells was studied. METHODS: A strain of
       mycoplasma was isolated from HIV- and HTLV-I-infected human helper
       T-cell line. Lipids extracted from the Mycoplasma fermentans and various
       mycoplasma species were added to latently HIV-infected macrophage cells
       (J22HL60). The expression of HIV antigens was analyzed by
       immunofluorescence flow cytometer using anti-HIV serum, and ELISA for
       the detection of p24 gag protein of HIV. RESULTS: We found an novel
       phosphorylcholine-containing glycoglycerolipid in the mycoplasma from
       human helper T-cell culture, and determined the structure of it. We
       could isolate one species of mycoplasma and identified it as Mycoplasma
       fermentans. The lipid fraction of the Mycolasma fermentans (named
       GGPLstrain) showed activity to induce HIV-antigen in J22HL60 cells, but
       lipid fractions from the other mycoplasma species did not. DISCUSSION
       AND CONCLUSIONS: Our data suggest a possible involvement of Mycolasma
       fermentans in AIDS pathogenesis. Since the novel lipid was specifically
       detected in Mycoplasma fermentans species, the lipid seems to be
       involved in the mechanism of HIV induction.
 DE    Cell Line  Enzyme-Linked Immunosorbent Assay  Flow Cytometry/METHODS
       Fluorescent Antibody Technique  Glycolipids/ISOLATION &
       PURIF/PHARMACOLOGY/*PHYSIOLOGY  Human  HIV/DRUG EFFECTS/*PHYSIOLOGY  HIV
       Antigens/BIOSYNTHESIS  HIV Core Protein p24/ANALYSIS/BIOSYNTHESIS
       HTLV-I/DRUG EFFECTS/*PHYSIOLOGY  Macrophages  Mycoplasma
       fermentans/ISOLATION & PURIF/*PHYSIOLOGY  Phosphorylcholine/ISOLATION &
       PURIF/*METABOLISM/PHARMACOLOGY  T-Lymphocytes,
       Helper-Inducer/*MICROBIOLOGY  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).