Document 3156
 DOCN  M94A3156
 TI    Enhancement of HIV-1 expression with lipopolisaccharide (LPS).
 DT    9412
 AU    Sanuma K; Chowdhury H; Matsuda K; Koyanagi Y; Yamamoto N; Department of
       Microbiology, Tokyo Medical and Dental University.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):140 (abstract no. PA0178). Unique
       Identifier : AIDSLINE ICA10/94369419
 AB    OBJECTIVE: Using CD14-positive HIV-1 infected cell line, J22HL60, we try
       to find out the mechanism of HIV expression induced by LPS in
       macrophage. METHODS: Induction of J22HL60 cells was proceeded for HIV
       expression with or without inhibitors. HIV expression was detected by
       indirect immunofluorescent staining and P24 assay. Concentration of
       diacylglycerol (DAG) was measured with production of radiorabeled
       compound by DAG kinase. RESULTS: Not only LPS but olso DAG was a potent
       HIV inducer. Staurosporine as well as anti-CD14 antibody supressed HIV
       induction. LPS stimulation increased DAG concentration in cell membrane.
       DISCUSSION AND CONCLUSIONS: Protein kinase C and phospholipase C (PLC)
       are involved in HIV expression in macrophage. CD-14 could transduce a
       signal toward PLC.
 DE    Alkaloids/PHARMACOLOGY  Antigens, CD/PHYSIOLOGY  Antigens,
       Differentiation, Myelomonocytic/PHYSIOLOGY  Cell Line
       Diglycerides/METABOLISM  Fluorescent Antibody Technique  Human  HIV Core
       Protein p24/ANALYSIS/BIOSYNTHESIS  HIV-1/DRUG EFFECTS/*PHYSIOLOGY
       Lipopolysaccharides/*PHARMACOLOGY  Macrophages  Phospholipase
       C/METABOLISM  Protein Kinase C/ANTAGONISTS & INHIB/METABOLISM  Signal
       Transduction  Virus Replication/*DRUG EFFECTS  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).