Document 3161
 DOCN  M94A3161
 TI    The role of human CD4 and CD26 in HIV infection of murine and rabbit
       transfectants.
 DT    9412
 AU    Yamamura Y; Ikawa Y; Dept. Biochemistry, Tokyo Medical & Dental Univ.
       Sch. Medicine,; Japan.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):139 (abstract no. PA0176). Unique
       Identifier : AIDSLINE ICA10/94369414
 AB    OBJECTIVE: Human CD4 is essential for HIV binding but not for HIV entry
       into the cells to establish infection. Although the human CD26 molecule
       was recently reported to be a cofactor that helps HIV infection, it is
       unclear whether only human CD4 and CD26 are sufficient for HIV
       infection. We evaluate the roles of human CD4 and CD26 in HIV-1 binding,
       entry, replication, and infection using murine and rabbit T cells and
       discuss the possibility of mice and rabbits as animal models for HIV
       infection. METHODS: Human CD4 and CD26 cDNAs were inserted into an
       expression vector BCMGSNeo and introduced into murine and rabbit T
       cells. The transfectants expressing human CD4 and CD26 were examined for
       susceptibility to HIV-1 by incubating with HIV-1 or by cocultivating
       with HIV-1-infected MOLT-4 cells. RESULTS: Rabbit but murine
       transfectans expressing human CD4 transiently expressed HIV-specific
       antigens after the HIV-1 infection. After the cell-mediated HIV-1
       infection, prominent syncytia formation together with the production of
       the HIV capsid protein, p24 and budding virions was observed in rabbit
       transfectants expressing human CD4 but was not in murine transfectants.
       Furthermore, data will be presented using murine and rabbit
       transfectants expressing both human CD4 and CD26. Preliminarily, murine
       transfectants expressing both human CD4 and CD26 were not susceptible to
       HIV-1 infection. DISCUSSION AND CONCLUSIONS: Our results suggest that
       only human CD4 and CD26 are not sufficient for HIV infection. Rabbit T
       cells might have or might be provided by MOLT-4 cells with the third
       molecule which is required for HIV infection.
 DE    Animal  Antigens, CD/BIOSYNTHESIS/*PHYSIOLOGY  Antigens,
       CD4/BIOSYNTHESIS/*PHYSIOLOGY  Antigens, Differentiation,
       T-Lymphocyte/BIOSYNTHESIS/*PHYSIOLOGY  Cell Line  Giant Cells  Human
       HIV-1/*PHYSIOLOGY  Mice  Rabbits  Recombinant
       Proteins/BIOSYNTHESIS/METABOLISM  T-Lymphocytes/*IMMUNOLOGY/MICROBIOLOGY
       *Transfection  Viral Proteins/BIOSYNTHESIS  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).