Document 3167
 DOCN  M94A3167
 TI    Functional analysis of retroviral env peptide recognized by T helper
       clones.
 DT    9412
 AU    Yamagishi H; Shimizu T; Uenishi H; Teramura Y; Iwashiro M; Kuribayashi
       K; Dept. Biophysics, Fac. Science, Kyoto University, Japan.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):138 (abstract no. PA0172). Unique
       Identifier : AIDSLINE ICA10/94369408
 AB    OBJECTIVE: We studied peptide-MHC interactions and their subsequent
       recognition by murine retroviral env122-141 specific Th cells. METHODS:
       We used the target peptide of decreasing length to define the preferred
       size of peptide and the variant peptide with single alanine
       substitutions to identify key amino acids required for binding to MHC
       molecules and for stimulating Th clones in vitro. RESULTS: Systematic
       analysis defined the minimum core length of 13 amino acids
       (LTSLTPRCNTAWN). Th clones were different in reactivity toward varying
       peptide length and the variant peptides. Peptide analog possessing
       minimum requirement for length of 13 residues and 5 key contact residues
       did not initiate full T-cell signaling of a high responsive clone but
       restored full T-cell activity when linked to an octavalent antigen
       peptide system (MAPS). DISCUSSION AND CONCLUSIONS: Eight dendritic arms
       of MAP-peptide may increase the local density of TcR-MHC complexes to
       lead to T cell activation. Thus, the MAP-peptide may provide a powerful
       tool in eliciting retroviral peptide specific T helper responses.
 DE    Amino Acid Sequence  Animal  Clone Cells  Comparative Study  Gene
       Products, env/*IMMUNOLOGY  Lymphocyte Transformation  Major
       Histocompatibility Complex  Mice  Molecular Sequence Data
       Oligopeptides/CHEMICAL SYNTHESIS/IMMUNOLOGY  Peptide Fragments/CHEMICAL
       SYNTHESIS/*IMMUNOLOGY  Retroviridae/*IMMUNOLOGY  Structure-Activity
       Relationship  T-Lymphocytes/*IMMUNOLOGY  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).