Document 3168
 DOCN  M94A3168
 TI    Analysis of Gag protein region involved in feline immunodeficiency virus
       particle formation.
 DT    9412
 AU    Morikawa S; Kitamura T; National Institute of Health, Tokyo, Japan.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):138 (abstract no. PA0171). Unique
       Identifier : AIDSLINE ICA10/94369407
 AB    OBJECTIVE: To elucidate the functional domains required for proper
       assembly to Gag precursor proteins of feline immunodeficiency virus
       (FIV). METHODS: A variety of deletions were introduced within the FIV
       gag gene. Several chimeric gag genes were constructed between FIV and
       simian immunodeficiency virus (SIV). These recombinant genes were
       expressed in insect cells by recombinant baculoviruses and analyzed
       their potential to assemble into particles. RESULTS: 1) deletion of
       either MA or NC proteins abolished particle formation of FIV Gag
       precursor; 2) N'-terminal region including two Cys-His boxes of FIV NC
       protein is necessary for assembly; 3) MA and NC protein regions of FIV
       Gag precursor can be functionally replaced by corresponding regions of
       SIV. DISCUSSION AND CONCLUSIONS: Two functional domains for the assembly
       of FIV Gag precursor into particles has been identified. These domains
       can be functionally complemented by corresponding domains of SIV. The
       results suggest that the assembly domains of Gag is functionally
       conserved among lentiviruses even though there is little amino acid
       sequence homology in these domains.
 DE    Animal  Baculoviridae  Base Sequence  Cell Line  Chimera  Comparative
       Study  Conserved Sequence  Gene Products, gag/*BIOSYNTHESIS  *Genes, gag
       Immunodeficiency Virus, Feline/*GENETICS/PHYSIOLOGY  Insects
       Lentivirus/GENETICS  Recombinant Proteins/BIOSYNTHESIS  Recombination,
       Genetic  *Sequence Deletion  SIV/*GENETICS/PHYSIOLOGY  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).