Document 3169
 DOCN  M94A3169
 TI    Protein tyrosine phosphorylation in HTLV-1 infected and Tax1-transduced
       T lymphocytes.
 DT    9412
 AU    Akagi T; Tono H; Shimotohno K; Virology Division, National Cancer Center
       Research Institute,; Tokyo, Japan.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):137 (abstract no. PA0166). Unique
       Identifier : AIDSLINE ICA10/94369406
 AB    To elucidate the mechanism of abnormal growth of HTLV-1 infected T
       lymphocytes, we have investigated the protein tyrosine-phosphorylation,
       which is known to play an important role in growth-signal transduction,
       by the western blot analysis using anti-phosphotyrosine antibody. HTLV-1
       infected T cell clone showed significantly elevated level of protein
       tyrosine-phosphorylation compared to control T cell clone.
       Tyrosine-phosphorylated proteins of 64kd and 35kd were observed
       specifically in HTLV-1 infected T cell clone. Tyrosine-phosphorylation
       of 64kd protein was also observed in Tax1-transduced T cells which were
       derived from PBL by using a retroviral vector. These elevated levels of
       protein tyrosine-phosphorylation possibly play some role in the abnormal
       proliferation of HTLV-1 infected T lymphocytes.
 DE    Blotting, Western  Clone Cells  Comparative Study  Gene Products,
       tax/*METABOLISM  Human  HTLV-I/*PHYSIOLOGY  Lymphocyte Transformation
       Phosphoproteins/ISOLATION & PURIF/*METABOLISM  Phosphorylation
       Recombinant Proteins/METABOLISM  *Signal Transduction
       T-Lymphocytes/*PHYSIOLOGY  Transfection  Tyrosine/*ANALOGS &
       DERIVATIVES/METABOLISM  MEETING ABSTRACT

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