Document 3170
 DOCN  M94A3170
 TI    Properties of a defective SIVsm virus clone.
 DT    9412
 AU    Kraiselburd E; Malaga C; Beltran M; Leung N; Torres J; UPR Med Sch.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):137 (abstract no. PA0168). Unique
       Identifier : AIDSLINE ICA10/94369405
 AB    OBJECTIVE: To determine the properties of Viruses produced by an SIVsm
       chronically infected cell line. METHODS: Particles were examined by
       e.m., by infectivity assays (in vitro and in vivo) and by immunoblots.
       Nucleic acid was partially sequenced. RESULTS: Defective particles were
       immature. The contained env and gag proteins but lacked vpx and vpr. A
       1.6 kb genome deletion was observed. Particles were not infectious yet
       they were immunogenic, and elicited neutralizing antibodies when
       inoculated with and adjuvant to naire rhesus macaques. DISCUSSION AND
       CONCLUSION: The approximately 1.6 kb deletion possibly involves
       integrase, vif, vpr, and vpx. Some of these enzymes are required for
       viral replication in vitro as well as in vivo. This immunogenic
       particles could be used as a model for the study of vaccines against
       pathogenic SIV.
 DE    Animal  Cell Line  Defective Viruses/GENETICS/*PHYSIOLOGY/PATHOGENICITY
       DNA Nucleotidyltransferases/METABOLISM  *Gene Deletion  Gene Products,
       env/*BIOSYNTHESIS  Gene Products, gag/*BIOSYNTHESIS  Genes, env  Genes,
       gag  Genes, vpr  *Genes, Viral  Genome, Viral
       SIV/GENETICS/*PHYSIOLOGY/PATHOGENICITY  Virus Replication/PHYSIOLOGY
       MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).