Document 3171
 DOCN  M94A3171
 TI    Inverse correlation between viremia and natural killer cells in a
       naturally SIVcpz-ant infected chimpanzee.
 DT    9412
 AU    Kestens L; Vingerhoets J; Peeters M; Vanham G; van der Groen G; Gigase
       P; Heeney J; Inst. of Trop. Med., Antwerpen, Belgium.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):137 (abstract no. PA0169). Unique
       Identifier : AIDSLINE ICA10/94369404
 AB    OBJECTIVE: 1) To analyze parameters of cellular immunity in a
       wild-caught chimpanzee, naturally infected with an HIV-1 related
       lentivirus (SIVcpz-ant). 2) To compare results of 36 months of follow-up
       with 3 experimentally HIV-1 infected and 5 uninfected control
       chimpanzees. METHODS: Quantitative virus cultures from peripheral blood
       mononuclear cells (PBMC) and plasma, and parameters of cellular immunity
       were assessed quarterly. Natural killer (NK) cells (CD3-CD8+) and CD4
       cells were enumerated in peripheral blood by flow cytometry. NK function
       was assessed in a Cr-51 release assay. In vitro suppression of virus
       replication by PBMC was assessed after removal of CD8+ cells and CD3+
       cells respectively. RESULTS: In the SIVcpz-ant-infected chimpanzee, no
       clinical immunodeficiency was observed during 36 months follow-up
       period. Virus could always successfully be cultured from PBMC with
       relative stable virus titers. In plasma, titers of infectious virus
       fluctuated and ranged from < 1 to 500 TCID/ml plasma. During follow up,
       the absolute CD4 count remained always within the range of uninfected
       chimpanzees. The absolute number of NK cells varied significantly during
       follow-up (200 to 3000 cells/microliter) and tended to correlate
       inversely with the virus titer in blood or plasma. CD8+ T cells, but not
       CD8+ NK cells, were found to exert a significant suppressive activity on
       virus replication in vitro. CONCLUSION: Our data indicate that CD8+
       lymphocytes, and CD8+ T cells in particular have a role in controlling
       the persistent viremia. In addition, the results are suggestive for an
       interaction between SIVcpz-ant and NK cells in the naturally infected
       chimpanzee.
 DE    Animal  Animals, Wild  Antigens, CD3/ANALYSIS  Antigens, CD8/ANALYSIS
       Chimpansee troglodytes  Flow Cytometry  Follow-Up Studies  Immunity,
       Cellular  Killer Cells, Natural/*IMMUNOLOGY  Simian Acquired
       Immunodeficiency Syndrome/*IMMUNOLOGY  *SIV  T-Lymphocyte
       Subsets/*IMMUNOLOGY  T4 Lymphocytes/IMMUNOLOGY  Viremia/*IMMUNOLOGY
       MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
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