Document 3172
 DOCN  M94A3172
 TI    Expression of HTLV-I rex and tax recombinant proteins in E. coli.
 DT    9412
 AU    Susova O; Pavlish O; Scherbak L; Gurtsevitch V; Cancer Research Center,
       Moscow, Russia.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):137 (abstract no. PA0167). Unique
       Identifier : AIDSLINE ICA10/94369403
 AB    OBJECTIVE: HTLV-I has been known to contain specific regulatory genes
       rex and tax. The aim of the investigation was to obtain and to
       characterize recombinant proteins encoded by these genes in bacterial
       cells. METHODS: Routine gene-engeneering and biotechnological procedures
       were used to accomplish molecular cloning for above purposes. Several
       kind of expressing vectors were used including pATH, pEX as well as
       pMAL-c and pMAL-p which were described elsewhere. RESULTS:
       pMAL-c-derived plasmid clone expressing C-terminal part of p40-tax (167
       aa residues) turned out to be the most perspective for both to generate
       anti-tax rabbit polyclonal antibodies and to detect tax-specific
       antibodies in human sera. Recombinant p21-rex hybrid protein which has
       been expressed in pATH-vector system was low immunogenic and incapable
       to detect rex-specific antibodies in human sera of HTLV-I carriers.
       However, rabbit antiserum obtained against mentioned recombinant protein
       was able to detect p21 cellular protein in HTLV-I-containing cell lines
       (C 91/PL, MT-2, HVT-102). CONCLUSIONS: A new plasmid DNAs expressing
       HTLV-I recombinant tax and rex proteins and generating appropriate
       polyclonal anti-sera have been obtained. Immunoblot analysis allowed us
       to demonstrate a moderate immune response to p40-tax in some cases with
       no antibodies to HTLV-I structural proteins.
 DE    Cloning, Molecular/METHODS  DNA, Viral/METABOLISM  Gene Products,
       rex/*BIOSYNTHESIS  Gene Products, tax/*BIOSYNTHESIS  Genes, pX  Genetic
       Vectors  HTLV-I/GENETICS/*METABOLISM  Plasmids  Recombinant
       Proteins/*BIOSYNTHESIS  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).