Document 3177
 DOCN  M94A3177
 TI    Roles of HTLV-1 and genetic background in rat model of HAM/TSP.
 DT    9412
 AU    Kushida S; Mizusawa H; Tanaka H; Ami Y; Hori M; Yagami K; Kameyama T;
       Yoshida A; Nyunoya H; Iwasaki Y; et al; Institute of Basic Medical
       Sciences, University of Tsukuba,; Ibaraki, Japan.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):135 (abstract no. PA0161). Unique
       Identifier : AIDSLINE ICA10/94369398
 AB    OBJECTIVE: Clarification of the roles of Human T-cell leukemia virus
       type-1 (HTLV-1) in HTLV-1-associated myelopathy (HAM)/tropical spastic
       paraparesis (TSP) with rat model of HAM/TSP. METHODS: HTLV-1-producing T
       cells were injected intravenously into WKA and F344 rats. Detection of
       HTLV-1 provirus in peripheral blood mononuclear cells and organs of the
       rats was performed by PCR. Titers of anti-HTLV-1 gag and envelope
       antibodies were analyzed by particle agglutination kit and ELISA,
       respectively. RESULTS: Six out of the eight WKA rats injected with
       HTLV-1-producing T cells showed HAM/TSP-like paraparesis, while no
       symptoms were observed in seven F344 rats injected with HTLV-1-producing
       T cells or eight untreated WKA rats. The antibody response against
       envelope protein was lower in WKA rats than in F344 rats. White matter
       degeneration with infiltration of macrophages was found in the spinal
       cord and peripheral nerves. DISCUSSION AND CONCLUSIONS: This study
       showed the importance of HTLV-1 infection and the genetic background of
       the host in the neuropathogenesis of the HAM/TSP-like rats.
 DE    Animal  Comparative Study  Disease Models, Animal  Gene Products,
       gag/IMMUNOLOGY  HTLV-I/*ISOLATION & PURIF  HTLV-I Antibodies/BLOOD
       HTLV-I Infections/IMMUNOLOGY/*PHYSIOPATHOLOGY  Lymphocytes/MICROBIOLOGY
       Paraparesis, Tropical Spastic/IMMUNOLOGY/*PHYSIOPATHOLOGY
       Proviruses/ISOLATION & PURIF  Rats  Rats, Inbred F344/GENETICS  Rats,
       Inbred Strains/GENETICS  Species Specificity  Viral Envelope
       Proteins/IMMUNOLOGY  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).