Document 3186
 DOCN  M94A3186
 TI    HIV/HTLV western blot cross reactivity.
 DT    9412
 AU    Constantine NT; Jones KS; Zhang X; Abdel-Hamid M; Dept. Pathology, Univ.
       of Maryland Sch. of Med., Baltimore 21201.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):133 (abstract no. PA0150). Unique
       Identifier : AIDSLINE ICA10/94369389
 AB    OBJECTIVES: To assess the cross reactivity between HIV and HTLV by
       Western blots (WB). METHODS: 100 sera, 92 classified as HIV inconclusive
       (ELISA reactive but not confirmed by HIV WB), and 8 WB+ were tested
       using one of two HTLV WB (1.2, Diagnostic Biotechnology; or HTLV-I/II,
       Cambridge Biotech). 30 sera had been negative by HIV WB, while 62 had
       produced indeterminate results. RESULTS: None of the sera tested were
       confirmed positive for HTLV by WB. However, 19/92 (20%) of sera from
       individuals with indeterminate HIV status showed cross reactivity with
       HTLV. Cross reactivity was observed in 25% (16/62) of the HIV ELISA+/WB
       indeterminate sera and 10% (3/30) of the ELISA+/WB negative sera. None
       of the sera from HIV WB+ individuals exhibited cross reactivity to HTLV.
       Among patients with reactivity to both HIV and HTLV, 11/16 (69%) reacted
       to gag proteins from both viruses, while 0/16 reacted to env proteins
       from both viruses. DISCUSSION AND CONCLUSIONS: We have previously
       observed that HIV WB+ sera do not usually recognize HTLV proteins by WB.
       Therefore, it appears that cross reactivity observed in sera from
       individuals who are indeterminate for both viruses by WB are not due to
       the recognition of HTLV proteins by antibodies against HIV. Possible
       explanations include: (1) non viral antigens with epitopes similar to
       retroviral proteins, and (2) expression of endogenous retroviral
       sequences.
 DE    Blotting, Western/*METHODS  Comparative Study  Cross Reactions
       Enzyme-Linked Immunosorbent Assay/METHODS  Gene Products, gag/IMMUNOLOGY
       Human  HIV/ISOLATION & PURIF  HIV Antibodies/*BLOOD  HIV
       Infections/BLOOD/*DIAGNOSIS/IMMUNOLOGY  HTLV Antibodies/*BLOOD  HTLV
       Infections/BLOOD/*DIAGNOSIS/IMMUNOLOGY  HTLV-BLV Viruses/ISOLATION &
       PURIF  Predictive Value of Tests  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).