Document 3187
 DOCN  M94A3187
 TI    Evaluation of immunoassays for detection of HTLV-I/II antibodies.
 DT    9412
 AU    Andersson S; Godoy K; Thorstensson R; Dias F; Biberfeld G; Dept. of
       Immunology, Swedish Institute for Infectious Disease; Control,
       Stockholm, Sweden.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):133 (abstract no. PA0151). Unique
       Identifier : AIDSLINE ICA10/94369388
 AB    BACKGROUND: A general screening of blood donors for HTLV-I/II infection
       during one year from the beginning of 1994 has been initiated in Sweden.
       OBJECTIVE: To assess the sensitivity and specificity of commercially
       available screening assays for HTLV-I/II antibodies. METHODS: Serum
       panels (Swedish) 500-628 unselected fresh blood donor sera, 400
       unselected frozen blood donor sera, 12 selected HTLV-II positive sera
       from IVDUs. West African (Guinea-Bissau) 400 unselected frozen sera from
       medical and surgical in-patients at the National Hospital, Bissau (347
       negative, 22 HTLV-I positive and 31 ID (excluded)), 79 selected HTLV-I
       positive sera and 6 selected HTLV-II positive sera. The performance of 5
       different ELISA tests (see below) were evaluated. RESULTS: The
       sensitivity for HTLV-I was 100% for all assays. The Murex/Cambridge EIA
       detected all 18 HTLV-II samples. The other 4 assays missed one HTLV-II
       positive sample each after repeated testing (initially 1-4 false
       negative results). Organon missed one Swedish HTLV-II positive sample,
       all other false negative HTLV-II positive samples were from Africa.
       TABULAR DATA, SEE ABSTRACT VOLUME. CONCLUSION: The sensitivity for
       HTLV-I and the specificity was high for all assays. The results indicate
       that HTLV-II seropositive sera may give false negative results on some
       assays. Further characterization of the HTLV-II seropositive samples
       with PCR and virus isolation is under way and may give more light to the
       results obtained. Evaluations of other assays for detection of
       antibodies to HTLV are ongoing and the results will be presented.
 DE    Blood Donors  Blood Specimen Collection  Comparative Study
       Enzyme-Linked Immunosorbent Assay/*METHODS  False Negative Reactions
       Guinea-Bissau  Human  HTLV-I Antibodies/*BLOOD  HTLV-II
       Antibodies/*BLOOD  HTLV-II Infections/BLOOD/*DIAGNOSIS/IMMUNOLOGY
       Inpatients  Polymerase Chain Reaction/METHODS  Sensitivity and
       Specificity  Sweden  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).