Document 3189
 DOCN  M94A3189
 TI    The growth potency and cytopathogenicity of recombinant chimeric viruses
       between HIV-1 and SIVagm.
 DT    9412
 AU    Jin MH; Ido E; Kuwata T; Igarashi T; Okada M; Cichutek K; Kurth R; Miura
       T; Hayami M; Inst. for Virus Res., Kyoto Univ., Japan.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):132 (abstract no. PA0146). Unique
       Identifier : AIDSLINE ICA10/94369386
 AB    OBJECTIVE: We attempted to identify the viral determinant for
       pathogenicity by constructing recombinant chimeric viruses between HIV-1
       and a non-pathogenic SIVagm originally isolated from African green
       monkeys. METHODS: Starting from two infectious DNA clones, pNL432
       (HIV-1) and pSIVagm3 (SIVagm3), two recombinant chimeric viruses have
       been constructed. One of the chimeras, designated as HE-A391, has
       HIV-1-derived env region (including tat, rev, vpu) with the rest of its
       genome from SIVagm3; another, designated as SE-H13, has reciprocally
       SIVagm3-derived tat, rev and env in the genome of HIV-1. RESULTS: Both
       chimeric constructs yielded infectious viruses replicable in CD4
       positive human cell line, M8166. Western blotting analysis revealed that
       their structural proteins of both chimeric viruses were expressed as
       designed. SE-H13 and HIV-1 were highly growth-competent with severe
       cytopathic effect (CPE) in human PBMC whereas HE-A391 and SIVagm were
       weakly replicable with no CPE. Similar results were obtained in other
       human cell lines. On the other hand, the growth potency and
       cytopathogenicity of the viruses were different in monkey PBMC where
       SIVagm3 and HE-A391 were replicable with no CPE and HIV-1 and SE-H13
       were weakly or only transiently replicable. In the case of SE-H13, not
       CPE but severe cell deaths were observed. DISCUSSION AND CONCLUSIONS:
       These results seem to suggest that the 5' half region of the virus
       genome (LTR, gag and pol) is mainly important for the cytopathogenicity
       as well as the cell tropism. These HIV-1/SIVagm3 chimeras will be useful
       a variety of AIDS pathogenesis and vaccine studies.
 DE    Animal  Cell Death  Cell Line  Cercopithecus aethiops  Chimera
       Comparative Study  Genes, env  Genes, rev  Genes, tat  Genome, Viral
       Human  HIV Long Terminal Repeat
       HIV-1/GENETICS/*PHYSIOLOGY/*PATHOGENICITY  Lymphocytes/MICROBIOLOGY
       *Recombination, Genetic  Repetitive Sequences, Nucleic Acid
       SIV/GENETICS/*PHYSIOLOGY/*PATHOGENICITY  Virus Replication  MEETING
       ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).