Document 3197
 DOCN  M94A3197
 TI    The effect of glycyrrhizin in mice infected with murine retrovirus. II.
       Inhibitory effects on proliferation and function of lymphoid cells.
 DT    9412
 AU    Watanabe H; Miyaji C; Makino M; Abo T; Dep. of Immuno., Niigata Univ.
       Sch. of Med., Japan.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):130 (abstract no. PA0140). Unique
       Identifier : AIDSLINE ICA10/94369378
 AB    OBJECTIVE: Glycyrrhizin (GL) was found to be effective in HIV infected
       patients as shown by improvements of their clinical symptoms and of
       their immunological and liver function. GL activated selectively
       extrathymic T cells in the liver, and it is suspected that this
       phenomenon might be intimately related to such the clinical effect. In
       this studies, we investigated as to its effects on T cells of mice,
       murine AIDS (MAIDS) model. METHODS: C57BL/6 mice were inoculated with
       LP-BM5 MuLV. Treatment with GL was began 0 day or 4 weeks after the
       virus inoculation, three times a week. The cell surface phenotypes in
       various lymphoid organs were analyzed by flowcytometry. The production
       of cytokines of spleen cells and their responsiveness to IL-2 and SEB
       were also analyzed. RESULTS: A new method using anti-IL-2R beta chain
       MoAb and anti-CD3 MoAb could sensitively identify extrathymic T cells
       (CD3 intermediate and IL-2R beta +) in the liver. The cells from various
       organs in mice infected with the virus alone showed the phenotypic
       pattern of the expansion of extrathymic T cells. From these finding, we
       considered that the MuLV infection induced the over-activation of
       extrathymic T cells. Some mice treated with GL showed no signs of
       disease. GL had no effect on the restoration of IL-2 production and
       responsiveness to IL-2 and SEB, but effectively inhibited the abnormal
       production of IL-4, IFN-gamma and IL-6. CONCLUSIONS: These results
       suggested that GL had an ability to selectively control the
       over-activation of extrathymic T cells and the over-production of
       certain cytokines.
 DE    Animal  Antiviral Agents/*PHARMACOLOGY  Cytokines/*BIOSYNTHESIS  Flow
       Cytometry  Glycyrrhetinic Acid/*ANALOGS & DERIVATIVES/PHARMACOLOGY/
       THERAPEUTIC USE  Human  HIV Infections/DRUG THERAPY  Interferon Type
       II/BIOSYNTHESIS  Interleukin-2/PHARMACOLOGY  Interleukin-4/BIOSYNTHESIS
       Interleukin-6/BIOSYNTHESIS  *Leukemia Viruses, Murine  Lymphocyte
       Transformation/*DRUG EFFECTS  Lymphoid Tissue/DRUG EFFECTS/*IMMUNOLOGY
       Mice  Mice, Inbred C57BL  Murine Acquired Immunodeficiency
       Syndrome/*IMMUNOLOGY/PREVENTION  & CONTROL  T-Lymphocytes/DRUG
       EFFECTS/*IMMUNOLOGY  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).