Document 3204
 DOCN  M94A3204
 TI    Apoptosis induced in CD4+ cells expressing gp160 of HIV type 1.
 DT    9412
 AU    Koga Y; Sasaki M; Department of Infectious Disease, Tohkai University
       School of; Medicine, Kanagawa, Japan.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):13 (abstract no. 024A). Unique
       Identifier : AIDSLINE ICA10/94369371
 AB    CD4+ cell clones expressing gp160 or gp120 of HIV under the
       transcriptional control of an inducible promoter were made in order to
       examine the effect of these env products on the downregulation of
       surface CD4 and cell injury. A complete disappearance of surface CD4
       preceding single-cell death occurred in the cell clones expressing
       gp160, in which a complex between gp160 and CD4 was formed and
       accumulated intracellularly. In contrast with them, the cell clones
       expressing gp120 exhibited no such depletion of surface CD4 nor apparent
       cytopathic effect. Further investigation of the events that lead to cell
       death in such cells expressing gp160 demonstrated the induction of
       apoptosis in them. Prominent elevation of intracellular Ca2+ was
       observed at 10-15 min after the expression of gp160 in those cells, and
       the treatment of such cells with Ca2+ antagonists blocked significantly
       the induction of apoptosis, suggesting that Ca2+ is a critical signal
       transducing molecule leading to the apoptosis induced by gp160-CD4
       complex.
 DE    Antigens, CD4/DRUG EFFECTS  Antigens, Surface/DRUG EFFECTS
       Apoptosis/*DRUG EFFECTS  Calcium/METABOLISM  Cell Death/DRUG EFFECTS
       Gene Products, env/*PHARMACOLOGY  Human  HIV Envelope Protein
       gp120/PHARMACOLOGY  HIV-1/*PHYSIOLOGY  Protein Precursors/*PHARMACOLOGY
       T4 Lymphocytes/MICROBIOLOGY/*PHYSIOLOGY  MEETING ABSTRACT

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