Document 3205
 DOCN  M94A3205
 TI    Primary antibody response of human lymphocytes from SCID-hu mice
       injected with HIV1 peptides.
 DT    9412
 AU    Touraine JL; Chargui J; Desgranges C; Transplantation & Clinical
       Immunology Unit, INSERM U80, Lyon,; France.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):129 (abstract no. PA0135). Unique
       Identifier : AIDSLINE ICA10/94369370
 AB    OBJECTIVE: Severe combined immunodeficiency (SCID) mice can be
       transplanted with human lymphocytes (hu-PBL-SCID) or with human fetal
       liver, thymus and bone fragments (SCID-hu), thus providing a model for
       the study of HIV infection. This model has been used to investigate in
       vivo anti-HIV antibody response by human cells from such mice. METHODS:
       Hu-PBL-SCID and SCID-hu mice were injected with a variety of peptides
       from gp41 and antibodies were repeatedly checked by ELISA in mouse sera.
       RESULTS: Primary then secondary responses were shown to occur in SCID-hu
       mice, with 225 mg/l of human IgM and 300-1860 mg/l of human IgG. The
       antibody response was demonstrated to be primary in nature, particularly
       since the human cells derived from naive, fetal precursor cells. When
       hu-PBL-SCID mice received HIV peptides, only IgM anti-HIV antibodies
       were produced (372-424 mg/l); the switch to IgG antibodies did not
       occur, possibly due to the lack of human antigen-presenting cells (APC)
       in these mice injected with non-adherent PBL, in contrast with the
       normal development of APC from human stem cells in SCID-hu mice.
       DISCUSSION AND CONCLUSION: Such humanized mice therefore lend themselves
       to anti-HIV antibody production, including human monoclonal antibodies
       to HIV and they possibly will also contribute to preclinical evaluation
       of HIV candidate vaccines.
 DE    Animal  *Antibody Formation  Antigen-Presenting Cells/IMMUNOLOGY
       Comparative Study  Enzyme-Linked Immunosorbent Assay  Human  HIV
       Antibodies/*BIOSYNTHESIS/BLOOD  HIV Envelope Protein gp41/*IMMUNOLOGY
       IgG/BIOSYNTHESIS  IgM/BIOSYNTHESIS/BLOOD  *Lymphocyte Transfusion  Mice
       Mice, SCID  Peptide Fragments/IMMUNOLOGY  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).