Document 3206
 DOCN  M94A3206
 TI    Immune response and variation in provirus level during early phase of
       FIV-infected cats.
 DT    9412
 AU    Ohkura T; Iwa S; Tanabe-Tochikura A; Tamura T; Sato N; Kurimura T; Dept.
       of Pathology, Research Institute for Microbial Deseases,; Osaka
       University, Japan.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):129 (abstract no. PA0136). Unique
       Identifier : AIDSLINE ICA10/94369369
 AB    OBJECTIVE: To gain a better understanding of how the antibody in plasma
       and the provirus in PBMC vary in early phase of infection of SPF cats
       with FIV, we have analyzed the FIV and SPF cat system as an animal model
       of AIDS. METHODS: After i.v. inoculation of FIV (KYO-1 strain) to 5 SPF
       cats, the titer of antibody in plasma and the level of proviral DNA in
       PBMC was followed for 15 weeks. The total antibody response to FIV was
       analyzed by immunoprecipitation, and the level of total antibody was
       calculated from cpm of 35S labeled antigen bound with Protein A
       Sepharose CL4B beads. The anti-gp42 titer in plasma was further assayed
       with Vet Red Test (AGEN) by the mixture of plasma sample and red blood
       cells of a normal cat. Proviral DNA in PBMC was detected by PCR for gag
       region of FIV. RESULTS: Overt primary disease was not observed during
       the experiment, but anti-gp42 was first detected in week 1-2 after
       infection, aNd then the anti-gp42 titer gradually increased with the
       level of total antibody. The results of immunoprecipitation indicated
       the antibody to FIV env protein expressed at 2-3 weeks after infection.
       Proviral DNA was first detectable in week 2-3 after infection and then
       the DNA level evidently fluctuated. DISCUSSION AND CONCLUSIONS: By 3
       weeks after infection, proviral state in PBMC was established and then
       the level of proviral DNA in PBMC fluctuated significantly forming 2-3
       peaks up to the end of experimental period, but the titer of antigen to
       gp42 gradually increased after first detection of 1-2 weeks after
       infection. Those results indicate that the antibody response to gp42 is
       earlier than the first detection of proviral DNA, and then the titer of
       anti-gp42 elevated continuously suggesting antigen stimulation
       irrelevant of fluctuation in proviral DNA level.
 DE    Acquired Immunodeficiency Syndrome/MICROBIOLOGY  Animal  Antibodies,
       Viral/*BIOSYNTHESIS/BLOOD  Antibody Formation  Cats  Disease Models,
       Animal  DNA, Viral/*BLOOD  Feline Acquired Immunodeficiency
       Syndrome/*IMMUNOLOGY  Genes, gag  Human  Immunodeficiency Virus,
       Feline/IMMUNOLOGY/ISOLATION & PURIF/  *PHYSIOLOGY
       Lymphocytes/*MICROBIOLOGY  Polymerase Chain Reaction/METHODS
       Proviruses/IMMUNOLOGY/ISOLATION & PURIF/*PHYSIOLOGY  Virus Replication
       MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).