Document 3208
 DOCN  M94A3208
 TI    Adaptation of human immunodeficiency virus type 2 (HIV-2) primary
       isolates to the in vivo replication in macaque.
 DT    9412
 AU    Wakrim L; Nicol I; Le Grand R; Boussin F; Vaslin B; Roques P;
       Bayon-Auboyer MH; Dormont D; Laboratoire de Neuropathologie
       experimentale et Neurovirologie; CEA, CEN/FAR, DSV/DPTE/SSA,
       Fontenay-Aux-Roses, France.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):129 (abstract no. PA0134). Unique
       Identifier : AIDSLINE ICA10/94369367
 AB    OBJECTIVES: To obtain a human immunodeficiency virus type 2 isolate
       (HIV-2) reproductively and persistently infectious for macaque aiming to
       generate a suitable animal model for vaccine and therapeutic strategies.
       METHODS: Two macaques (macaca fascicularis) H100 and 306A were
       intravenously inoculated with respectively two HIV-2 isolates: HIV-2
       (33215), a primary isolate obtained from one HIV-2 ROD infected macaque
       which died from AIDS and PO-306A, a human HIV-2 primary isolate screened
       for its capacity to replicate in macaques PBMCs. Two successive
       transfusions of 10 ml whole blood were done from each of these macaques
       at early stages of infection into naive recipients (second and 3th in
       vivo passage). A 4th in vivo passage was done for the PO-306A isolate.
       Infection was monitored weekly by virus recovery from the PBMCs in
       coculture, by PCR, by P27 antigen detection and by seroconversion
       measured by ELISA and Western Blot. RESULTS: At each passage the animals
       were positive for virus isolation, PCR, and seroconversion. In the first
       transfused animal (second in vivo passage) which received the PO-306A
       isolate, infection was more severe and clinical signs like
       polyadenopathy and diarrhea were observed. All sera were negative for
       the P27 antigen detection by the P27-SIV Coulter kit except at the 4th
       passage. The virus isolated by coculture from monkey PBMCs at this later
       passage induces higher syncytia formation and higher level of reverse
       transcriptase activity. DISCUSSION: Taken together the importance and
       chronological appearance of different infection criteria, the PO-306A
       isolate seems to be adapted to replicate in macaque. The positive P27
       antigenemia obtained indicates that we can increase the level of in vivo
       replication by successive transfusions in naive recipients. CONCLUSION:
       In the attempt to generate a suitable animal model to study the
       infectivity, the pathogenicity and therapy the constitution of a virus
       stock is needed. Then the PO-306A virus isolate adaptated to replicate
       reproductively in macaque will be an interesting candidate.
 DE    Animal  Blood Transfusion  Disease Models, Animal  Giant
       Cells/IMMUNOLOGY  Human  HIV Antigens/ANALYSIS  HIV
       Seropositivity/IMMUNOLOGY  HIV-2/ISOLATION & PURIF/*PHYSIOLOGY
       Lymphocytes/IMMUNOLOGY/MICROBIOLOGY  Macaca fascicularis/*MICROBIOLOGY
       Polymerase Chain Reaction  Reagent Kits, Diagnostic  Reverse
       Transcriptase/ANALYSIS  *Virus Replication  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).