Document 3209
 DOCN  M94A3209
 TI    Vascular endothelial growth factor produced by cultured AIDS-associated
       Kaposi's sarcoma spindle cells.
 DT    9412
 AU    Sakurada S; Mori KM; Salahuddin SZ; Okamoto T; Nakamura S; Dept. of
       Molecular Genetics, Nagoya City University, Japan.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):128 (abstract no. PA0131). Unique
       Identifier : AIDSLINE ICA10/94369366
 AB    OBJECTIVE: To elucidate the mechanism of edema in AIDS-associated
       Kaposi's sarcoma (AIDS-KS) lesions. METHODS: Long-term cultured AIDS-KS
       spindle cells and their semi-purified conditioned medium (AIDS-KS CM)
       through heparin sepharose affinity column were tested to detect the
       activity inducing vascular hyper-permeability (VHP) in animal skin and
       promoting vascular endothelial cell growth in vitro. To identify the
       active agent produced by AIDS-KS cells, Western blot was done by using
       each fraction from the affinity column with anti-vascular endothelial
       growth factor(VEGF165) antibody and Northern blot was done by using RNA
       extracted from various AIDS-KS cell isolates with VEGF cDNA probe
       respectively. RESULTS: Both cultured AIDS-KS cells and AIDS-KS CM
       induced a specific histamine independent VHP in animal model and AIDS-KS
       CM demonstrated endothelial cell growth promoting activity in vitro. Two
       bands of 23.5 kD and 21.5 kD were identified in 0.5 M NaCl fraction by
       Western blot and high levels of VEGF mRNA in all AIDS-KS cell isolates
       by Northern blot. Moreover, the biological activity was totally
       neutralized by antibody to VEGF165. CONCLUSION: VEGF or VEGF like
       molecule was identified as the active agent causing significant
       complication of the disease: edema in AIDS-KS lesions.
 DE    Acquired Immunodeficiency Syndrome/*COMPLICATIONS  Animal  Blotting,
       Northern  Capillary Permeability/DRUG EFFECTS  Cell Division/DRUG
       EFFECTS  Cells, Cultured  Chromatography, Affinity  Culture Media,
       Conditioned  Endothelial Growth Factors/*BIOSYNTHESIS/ISOLATION & PURIF/
       PHARMACOLOGY  Endothelium, Vascular/CYTOLOGY/DRUG EFFECTS
       Histamine/PHARMACOLOGY  Human  Lymphokines/*BIOSYNTHESIS/ISOLATION &
       PURIF/PHARMACOLOGY  RNA, Neoplasm/ISOLATION & PURIF/METABOLISM  Sarcoma,
       Kaposi's/ETIOLOGY/*METABOLISM/*PATHOLOGY  Skin/BLOOD SUPPLY  Tumor
       Cells, Cultured  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).