Document 3211
 DOCN  M94A3211
 TI    Evidence for retroviral insertional mutagenesis in a subset of
       HIV-associated lymphomas.
 DT    9412
 AU    Herndier B; Shiramizu B; McGrath M; University of California, San
       Francisco 94110.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):128 (abstract no. PA0130). Unique
       Identifier : AIDSLINE ICA10/94369364
 AB    OBJECTIVE: To characterize on the molecular level a new class of
       HIV-associated lymphomas where the retrovirus HIV has inserted
       non-randomly into the tumor genomic DNA material. METHODS: 1) frozen
       section and paraffin section immunohistochemistry; 2) Southern blot
       analysis of inverse PCR products; 3) direct PCR sequencing; 4) mRNA
       analysis (Northern blots, cDNA-PCR). Abbreviations: Mo-macrophage;
       AILD-angioimmunoblastic lymphadenopathy. RESULTS: Inverse PCR (iPCR)
       uses the ability of restriction digests (Sau3a), circularization, and
       long terminal repeat primers to capture flanking sequences of the
       inserted retrovirus. Direct sequencing showed insertion of retroviral
       LTR sequence in exon Z of the fur fps/fes gene complex. The PCR products
       were subsequently probed with LTR and fur oligo's on Southern blots.
       TABULAR DATA, SEE ABSTRACT VOLUME. CONCLUSIONS: Fur fps/fes is a
       proto-oncogene complex (tyrosine kinase) activated in some myeloid
       tumors. The non-random (4 cases) clonal integration of HIV near this
       locus and subsequent differential activation of mRNA compared to control
       tissue (1 case) indicates that these lymphomas may have undergone
       retroviral insertional mutagenesis.
 DE    Acquired Immunodeficiency Syndrome/*COMPLICATIONS  Hodgkin's
       Disease/ETIOLOGY/GENETICS/MICROBIOLOGY  Human  HIV/*GENETICS  HIV Long
       Terminal Repeat  Lymphoma/ETIOLOGY/GENETICS/*MICROBIOLOGY  *Mutagenesis,
       Insertional  Oligonucleotide Probes  Polymerase Chain Reaction/METHODS
       Proto-Oncogenes  Restriction Mapping  Retroviridae/GENETICS/*ISOLATION &
       PURIF  RNA, Messenger/ANALYSIS  Virus Integration  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
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