Document 3218
 DOCN  M94A3218
 TI    Oral candidiasis in HIV infection--mucosal immunity versus strain
       selection.
 DT    9412
 AU    Challacombe SJ; Sweet SP; Dept. of Oral Medicine & Pathology, UMDS,
       Guy's Hospital, London,; UK.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):126 (abstract no. PA0124). Unique
       Identifier : AIDSLINE ICA10/94369357
 AB    OBJECTIVE: The aim of this study was to investigate the relative
       importance of host secretory immunity versus candidal pathogenicity in
       oral HIV-related candidiasis. METHODS: Whole and parotid saliva from HIV
       infected, AIDS and control subjects, and Candida sp. isolated from these
       groups, were compared. Five factors were analysed: total IgA
       concentrations; Candida IgA antibody levels; Candida biotype patterns;
       restriction fragment length polymorphism (RFLP) patterns; and adherence
       of Candida to buccal epithelial cells (BECs). Total IgA and Candida IgA
       antibody levels were determined by ELISA; biotypes with the API 20AUX
       system; RFLP analysis with BgIII restriction digests; and adherence with
       human BECs (10(5)/ml) and Candida (10(7)/ml) in vitro. RESULTS: Total
       IgA, IgA1 and IgA2 concentrations were lower in whole and parotid saliva
       in HIV and AIDS subjects compared with controls (P < 0.05), but Candida
       IgA, IgA1 and IgA2 antibody levels were higher (P < 0.05). More
       non-albicans species and more Candida biotypes were seen in the HIV/AIDS
       group. Among the C. albicans isolates, 11 unique genotypes were noted in
       the HIV/AIDS group compared with four from control subjects (P < 0.05)
       suggesting more genotypic as well as phenotypic variation. Almost all
       Candida species isolated from HIV or AIDS subjects adhered to BECs in
       higher numbers than control isolates (P < 0.001). DISCUSSION AND
       CONCLUSIONS: This study suggests that HIV infected and AIDS subjects
       maintain an adequate secretory immune response to Candida, and that the
       selection of strains of Candida with altered virulence determinants may
       account for the increased prevalence of candidiasis in HIV infection.
 DE    Antibodies, Fungal/*ANALYSIS  AIDS-Related Opportunistic
       Infections/IMMUNOLOGY/*MICROBIOLOGY
       Candida/*CLASSIFICATION/GENETICS/ISOLATION & PURIF  Candida
       albicans/CLASSIFICATION/GENETICS/ISOLATION & PURIF  Candidiasis,
       Oral/IMMUNOLOGY/*MICROBIOLOGY  Enzyme-Linked Immunosorbent Assay
       Genotype  Human  HIV Infections/IMMUNOLOGY/MICROBIOLOGY  IgA/*ANALYSIS
       Mouth Mucosa/IMMUNOLOGY/*MICROBIOLOGY  Parotid Gland/SECRETION
       Restriction Fragment Length Polymorphisms  Saliva/*MICROBIOLOGY  MEETING
       ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).