Document 3221
 DOCN  M94A3221
 TI    More apoptosis of T-cell subsets were observed in the more advanced
       HIV-infected individuals using BM-1/JIMRO mAb and flow cytometric
       analysis.
 DT    9412
 AU    Ruxrungtham K; Kashiwagi N; Adachi M; Sirivichayakul S; Maruyama I;
       Phanuphak P; Chulalongkom Hospital, Bangkok, Thailand.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):125 (abstract no. PA0119). Unique
       Identifier : AIDSLINE ICA10/94369354
 AB    BACKGROUND: Apoptosis is one of the proposed mechanisms of the depletion
       of CD4+ T-cells in AIDS. BM-1/JIMRO mAb recognizes Le(Y) carbohydrate
       antigen which is closely correlated with the process of apoptosis.
       OBJECTIVE: To analyse the expression of an apoptotic marker-BM-1 (Le(Y))
       Ag on peripheral blood T-cell subsets from HIV-infected individuals with
       different stages of the disease. METHODS: Heparinized blood was
       collected from 60 patients (12 Asymp, 7 PGL, 25 ARC, 16 AIDS) and 20 HIV
       seronegative individuals. 93% of the patients were sexually-acquired
       (87% Heterosexual) and 7% IVDU. Sex: M/F = 48/12. PBMC's were prepared
       by Ficoll hypaque separation. Cells were then double stained with
       CD4/CD8, BM-1/CD4, BM-1/CD8 and HLA-DR/CD8 and analyzed using flow
       cytometry. RESULTS: CD4+ T cells expressing BM-1 antigen was
       significantly elevated, but only in the patients with AIDS (p < 0.001,
       by Mann-Whitney). Interestingly, BM-1 Ag was highly expressed on CD8+ T
       cells from all groups of patients (PGL, ARC and AIDS, p < 0.05, < 0.05
       and < 0.001) except the asymptomatic. Whereas, HLA-DR+/CD8+ cells in all
       groups including the asymptomatic were significantly higher than the
       controls (p < 0.001, Mann-Whitney). DISCUSSION: In this preliminary
       study, more apoptosis of T-cell subsets (both CD4+ and CD8+) were
       observed in the more advanced HIV-infected patients than the controls.
       All groups of patients showed significantly high level of activated-CD8+
       (DR+) cells. Interestingly, in the asymptomatic patients revealed
       significantly high DR+/CD8+ but not BM-1+/CD8+ cells which suggests that
       BM-1 may not be an activation marker. BM-1 may be a valuable marker for
       the management of AIDS and may also shed more light in HIV pathogenesis.
 DE    *Antibodies, Monoclonal  Antigens, CD/BLOOD  Antigens, CD4/BLOOD
       Antigens, CD8/BLOOD  *Apoptosis  AIDS-Related Complex/BLOOD/IMMUNOLOGY
       Biological Markers/BLOOD  Cells, Cultured  Comparative Study  Female
       Flow Cytometry/*METHODS  Human  HIV Infections/BLOOD/*IMMUNOLOGY  HIV
       Seronegativity  HIV Seropositivity/BLOOD/*IMMUNOLOGY  Male  T-Lymphocyte
       Subsets/*PATHOLOGY  T4 Lymphocytes/*IMMUNOLOGY/PATHOLOGY  MEETING
       ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).