Document 3222
 DOCN  M94A3222
 TI    Interleukin 12 enhances host responses of HIV positive donors against
       Mycobacterium avium in vitro.
 DT    9412
 AU    Newman G; Guarnaccia J; Vance E; Remold H; Kazanjian P; Dept.
       Rheum/Immunol, Brigham & Women's Hospital/Harvard Medical; School,
       Boston, MA 02115.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):125 (abstract no. PA0121). Unique
       Identifier : AIDSLINE ICA10/94369353
 AB    OBJECTIVE: To determine whether IL-12 could enhance growth inhibition of
       an proliferative responses to M. avium by peripheral blood mononuclear
       cells (PMBC) from either HIV negative or positive donors. METHODS: PBMCs
       from 14 HIV+ and 12 HIV- donors were inoculated with M. avium, serovar
       4, and cultured for 7 days in the presence of IL-12. The cells were
       lysed and the amount of M. avium present determined by a radiometric
       broth assay. PBMCs from these same donors and 10 additional HIV+ donors,
       were also analyzed for their ability to proliferate to M. avium in the
       presence or absence of IL-12 by 3H-thymidine uptake. RESULTS: IL-12
       significantly enhanced (p = .001) the capacity of PBMCs to inhibit the
       growth of M. avium from all 12 HIV- donors, but only 5 of 14 HIV+
       donors. in contrast, IL-12 significantly (p = .0001) stimulated
       increased proliferation to M. avium by all donors regardless of HIV
       infection, although HIV- donors had a significantly greater response (p
       = .008) than HIV+ donors. IL-12 enhanced proliferation by HIV+ donors to
       M. avium was independent of CD4 counts. CONCLUSIONS: Exogenously
       administered IL-12 enhanced in vitro cellular responses to M. avium in
       both HIV+ and - donors. These results suggest that IL-12 by itself or
       used in conjunction with other cytokines may be of therapeutic value
       against M. avium infections in AIDS patients.
 DE    Cells, Cultured  Comparative Study  Growth Substances/*PHARMACOLOGY
       Human  HIV Seronegativity  HIV Seropositivity/*IMMUNOLOGY
       Interleukins/*PHARMACOLOGY  Lymphocyte Transformation/DRUG EFFECTS
       Lymphocytes/*DRUG EFFECTS/IMMUNOLOGY/MICROBIOLOGY  Mycobacterium
       avium/*DRUG EFFECTS/GROWTH & DEVELOPMENT  Reference Values
       Thymidine/METABOLISM  MEETING ABSTRACT

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