Document 3224
 DOCN  M94A3224
 TI    Cytofluorimetric and immunoelectronmicroscopic analysis of GM3
       expression on lymphocytes from HIV infected patients.
 DT    9412
 AU    Sorice M; Pavan A; Garofalo T; Circella A; d'Agostino F; Sansolini T;
       Pinter E; Lenti L; Torrisi MR; Dip. Med. Sperimentale, Universita La
       Sapienza, Roma, Italy.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):125 (abstract no. PA0120). Unique
       Identifier : AIDSLINE ICA10/94369351
 AB    Recently, we demonstrated (Misasi et al. Clin Immunol. Immunopathol.,
       67: 216, 1993) that lymphocytes from HIV infected patients have an
       increased concentration of GM3 as compared to lymphocytes from healthy
       donors or patients suffering from different viral diseases (infectious
       mononucleosis, acute B or chronic C hepatitis). In this report we
       investigated, using cytofluorimetric analysis and electron microscopy,
       the expression of GM3 on T cell plasma membrane of 25 HIV-infected
       patients. Cytofluorimetric analysis revealed a reactivity with anti-GM3
       monoclonal antibody (GMR-6, Kotani et al, Biochim. Biophys. Acta 1117:
       97, 1992) of 18.5 +/- 2.1% in the 25 anti-HIV+ subjects, as compared to
       7.2 +/- 2.4% in 40 matched healthy blood donors. In all subjects the
       reactivity was virtually the same in isolated CD4+ and CD8+ cells.
       Immunolabeling of GM3 at electron microscopic level, using the anti-GM3
       mAb followed by protein A colloidal gold conjugated, confirmed the
       increase of the GM3 expression in lymphocytes from HIV infected patients
       as compared to control cells. Further studies are in progress with the
       aim to analyze the pattern of distribution of GM3 on lymphocyte plasma
       membrane during HIV infection and to elucidate whether GM3 expression
       could represent an indicator of disease progression.
 DE    Antibodies, Monoclonal  Cell Membrane/METABOLISM/ULTRASTRUCTURE
       Comparative Study  Flow Cytometry/METHODS  G(M3)
       Ganglioside/*BIOSYNTHESIS/BLOOD  Human  HIV
       Infections/*IMMUNOLOGY/METABOLISM
       Lymphocytes/IMMUNOLOGY/*METABOLISM/ULTRASTRUCTURE  Microscopy,
       Immunoelectron/METHODS  T-Lymphocyte Subsets/METABOLISM  MEETING
       ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
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